Wu Min, Cai Ronghao, Liu Huanlong, Li Fei, Zhao Yang, Xiang Yan
National Engineering Laboratory of Crop Stress Resistance Breeding, Anhui Agricultural University, Hefei 230036, China.
Laboratory of Modern Biotechnology, School of Forestry and Landscape Architecture, Anhui Agricultural University, Hefei 230036, China.
Plant Cell Physiol. 2019 Jul 1;60(7):e1-e14. doi: 10.1093/pcp/pcy196.
Here, 10 drought-induced 19 (Di19) proteins from Phyllostachys edulis were analyzed and an important stress-related candidate gene (PeDi19-4) was isolated based on analysis of phylogenetic relationships and expression profiles. PeDi19-4 is a nuclear localization protein that can bind the conserved TACA(A/G)T sequence, as determined using enzyme-linked immunosorbent assay (EMSA). PeDi19-4 has no transcriptional activity in yeast but functions as a transcription activator in plants. Overexpression of PeDi19-4 in rice and Arabidopsis thaliana enhanced drought and salt tolerance as determined through phenotypic analysis and the use of stress-associated physiological indicators. PeDi19-4 transgenic plants showed increased sensitivity to ABA during seed germination and early seedling growth. Additionally, transgenic rice accumulated more ABA than wild-type plants under drought and salt stress conditions. Moreover, the stomata of PeDi19-4-overexpressing plants changed significantly with ABA treatment. RNA sequencing revealed that PeDi19-4 regulated the expression of a wide spectrum of stress-/ABA-responsive differentially expressed genes. The stress-responsive genes (OsZFP252 and OsNAC6) and ABA-responsive genes (OsBZ8 and OsbZIP23) were direct targets of PeDi19-4. Our research indicated that PeDi19-4 enhanced drought and salt tolerance in plants via the ABA-dependent signaling pathway.
在此，对毛竹中10种干旱诱导的19（Di19）蛋白进行了分析，并基于系统发育关系和表达谱分析分离出一个重要的胁迫相关候选基因（PeDi19-4）。通过酶联免疫吸附测定（EMSA）确定，PeDi19-4是一种可结合保守TACA(A/G)T序列的核定位蛋白。PeDi19-4在酵母中没有转录活性，但在植物中作为转录激活因子发挥作用。通过表型分析和使用胁迫相关生理指标确定，在水稻和拟南芥中过表达PeDi19-4可增强耐旱性和耐盐性。PeDi19-4转基因植物在种子萌发和幼苗早期生长期间对ABA的敏感性增加。此外，在干旱和盐胁迫条件下，转基因水稻比野生型植物积累更多的ABA。而且，用ABA处理后，过表达PeDi19-4的植物气孔发生了显著变化。RNA测序显示，PeDi19-4调节了广泛的胁迫/ABA响应差异表达基因的表达。胁迫响应基因（OsZFP252和OsNAC6）和ABA响应基因（OsBZ8和OsbZIP23）是PeDi19-4的直接靶标。我们的研究表明，PeDi19-4通过ABA依赖的信号通路增强了植物的耐旱性和耐盐性。
