Xu Zijian, Wang Fan, Ma Yongbo, Dang Haoran, Hu Xiaohui
College of Horticulture, Northwest AF University, Xianyang 712100, China.
Key Laboratory of Protected Horticultural Engineering in Northwest, Ministry of Agriculture, Xianyang 712100, China.
Antioxidants (Basel). 2022 Aug 27;11(9):1673. doi: 10.3390/antiox11091673.
Basic leucine zipper (bZIP) transcription factors of the ABA-responsive element binding factor/ABA-responsive element binding proteins (ABF/AREB) subfamily have been implicated in abscisic acid (ABA) and abiotic stress responses in plants. However, the specific function of ABF/AREB transcription factors under saline-alkaline stress is unclear. Here, we identified four ABF/AREB transcription factors in tomato and found that SlAREB1 strongly responded to both ABA and saline-alkaline stress. To further explore the function of SlAREB1 under saline-alkaline stress, SlAREB1-overexpressing lines were constructed. Compared with wild-type plants, SlAREB1-overexpressing transgenic tomato plants showed reduced malondialdehyde content, increased the relative water content, and alleviated the degradation of chlorophyll under saline-alkaline stress. Importantly, SlAREB1 directly physically interacted with SlMn-SOD, which improved the activity of antioxidant enzymes and increased the scavenging of excess reactive oxygen species. Overall, the overexpression of SlAREB1 increased the antioxidant capacity of the transgenic tomato under saline-alkaline stress.
脱落酸应答元件结合因子/脱落酸应答元件结合蛋白（ABF/AREB）亚家族的碱性亮氨酸拉链（bZIP）转录因子参与了植物中的脱落酸（ABA）和非生物胁迫应答。然而，ABF/AREB转录因子在盐碱胁迫下的具体功能尚不清楚。在此，我们在番茄中鉴定出四个ABF/AREB转录因子，并发现SlAREB1对ABA和盐碱胁迫均有强烈响应。为了进一步探究SlAREB1在盐碱胁迫下的功能，构建了过表达SlAREB1的株系。与野生型植株相比，过表达SlAREB1的转基因番茄植株在盐碱胁迫下丙二醛含量降低、相对含水量增加，并且叶绿素降解得到缓解。重要的是，SlAREB1与SlMn-SOD直接发生物理相互作用，这提高了抗氧化酶的活性并增强了对过量活性氧的清除。总体而言，SlAREB1的过表达提高了转基因番茄在盐碱胁迫下的抗氧化能力。
