巴西东北部人群中血清素和一氧化氮基因多态性与登革热临床结局的关联
Association of polymorphisms in serotonin and nitric oxide genes with clinical outcome of dengue in Brazilian northeast population.
作者信息
Dos Santos Ana Caroline Melo, de Moura Edilson Leite, da Silva Denise Macêdo, Araujo Moura Alexandre Wendell, Ferreira Jean Moises, Lira Neto Abel Barbosa, Pereira E Silva Aline Cristine, de Medeiros Alves Verônica, Balliano Tatiane Luciano, de Farias Karol Fireman, de Lima Filho José Luiz, de Souza Figueiredo Elaine Virgínia Martins
机构信息
Laboratorio de Biologia Molecular e Expressão Gênica (LABMEG), Universidade Federal de Alagoas, Av. Lourival Melo Mota, s/n Tabuleiro do Martins, CEP:57072-900, Campus A.C. Simões, Maceió, AL, Brazil.
Laboratorio de Biologia Molecular e Expressão Gênica (LABMEG), Universidade Federal de Alagoas, Campus Arapiraca, Av Manoel Severino Barbosa, Bom Sucesso, 57309-005, Arapiraca, AL, Brazil.
出版信息
Acta Trop. 2019 Feb;190:144-148. doi: 10.1016/j.actatropica.2018.11.015. Epub 2018 Nov 16.
Serotonin and nitric oxide seem to be involved in Dengue virus infection. The aim of this study was to investigate if SNPs in serotonin and nitric oxide are associated with dengue severity. A retrospective case-control study was conducted, with groups of dengue fever (DF; n = 78) and dengue hemorrhagic fever patients (DHF; n = 49). Genotyping was performed using qPCR and PCR. The power of the sample size was calculated by G*power software. The heterozygous SL for 5-HTTLPR SNP was significantly correlated with protection against progression to DHF in the codominant SS/SL/LL (OR = 0.22, 95% CI = 0.06-0.81, p = 0.011) and overdominant models SL vs SS + LL (OR = 0.19, 95% CI = 0.06-0.65, p = 0.003). For the ENOS (rs1799983) SNP, the genotype GT was positively associated with protection for development of the clinical form in DHF compared to dengue fever (OR = 0.39, 95% CI = (0.13-1.14), p = 0.0058) in codominant GG/GT/TT and overdominant model GT vs GG + TT (OR = 0.35, 95% CI = (0.12-1.02), p = 0.04). To our knowledge, this is the first study to identify the association of the serotonin and nitric oxide SNPs with dengue severity.
血清素和一氧化氮似乎与登革热病毒感染有关。本研究的目的是调查血清素和一氧化氮中的单核苷酸多态性（SNP）是否与登革热的严重程度相关。进行了一项回顾性病例对照研究，分为登革热组（DF；n = 78）和登革出血热患者组（DHF；n = 49）。使用定量聚合酶链反应（qPCR）和聚合酶链反应（PCR）进行基因分型。样本量的检验效能通过G*power软件计算。5-羟色胺转运体基因启动子区域（5-HTTLPR）SNP的杂合子SL在共显性模型SS/SL/LL（比值比[OR]=0.22，95%置信区间[CI]=0.06-0.81，p = 0.011）和超显性模型SL与SS + LL比较中（OR = 0.19，95% CI = 0.06-0.65，p = 0.003）与预防进展为登革出血热显著相关。对于内皮型一氧化氮合酶（ENOS，rs1799983）SNP，在共显性模型GG/GT/TT以及超显性模型GT与GG + TT比较中（OR = 0.35，95% CI = 0.12-1.02，p = 0.04），基因型GT与预防登革热发展为登革出血热临床类型呈正相关（与登革热相比，OR = 0.39，95% CI = 0.13-1.14，p = 0.0058）。据我们所知，这是第一项确定血清素和一氧化氮SNP与登革热严重程度之间关联的研究。
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