Polymers and Pigments Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, P.O. 12622, Egypt.
Polymers and Pigments Department, National Research Centre, 33 El Bohouth St., Dokki, Giza, P.O. 12622, Egypt.
Int J Biol Macromol. 2019 Feb 15;123:856-865. doi: 10.1016/j.ijbiomac.2018.11.155. Epub 2018 Nov 16.
Chitosan nanoparticles (CS-NPs) and chitosan/polyacrylic acid hydrogel nanoparticles (CS/PAA-HNPs) were obtained by ionic gelation with tripolyphosphate anions and copolymerization of CS with acrylic acid (AA), respectively. The prepared NPs were loaded by different concentrations of copper (1, 2 and 3% with respect to CS) to obtain chitosan/copper nanocomposites (CS/Cu-NCs) and chitosan/polyacrylic acid/copper hydrogel nanocomposites (CS/PAA/Cu-HNCs). The prepared NPs and their NCs were characterized by different techniques. The swelling properties and copper release from CS/Cu-NCs and CS/PAA/Cu-HNCs were evaluated. The antibacterial activity of the prepared samples against bacteria (e.g., Staphylococcus aureus and Pseudomonas aeruginosa), fungi, and yeast were investigated. The results displayed that the copper release, as well as the swelling percentage of CS/PAA/Cu-HNCs, were higher than that of CS/Cu-NCs. Furthermore, the impact of using CuSO, CS/Cu-NCs, and CS/PAA/Cu-HNCs as a different source of copper on chlorophyll content, vegetative growth, minerals content, and the yield of onion plants during two seasons 2016 and 2017 were studied. It was found that the yield, plant growth, and nutrient content of onion bulbs were improved using CS/PAA-HNPs, which was loaded with 75 ppm copper, as foliar spray for onion plants.
壳聚糖纳米粒子（CS-NPs）和壳聚糖/聚丙烯酸水凝胶纳米粒子（CS/PAA-HNPs）分别通过三聚磷酸阴离子的离子凝胶化和 CS 与丙烯酸（AA）的共聚反应获得。所制备的 NPs 用不同浓度的铜（相对于 CS 的 1%、2%和 3%）负载，以获得壳聚糖/铜纳米复合材料（CS/Cu-NCs）和壳聚糖/聚丙烯酸/铜水凝胶纳米复合材料（CS/PAA/Cu-HNCs）。通过不同的技术对所制备的 NPs 和它们的 NCs 进行了表征。评估了 CS/Cu-NCs 和 CS/PAA/Cu-HNCs 的溶胀性能和铜释放。研究了所制备的样品对细菌（如金黄色葡萄球菌和铜绿假单胞菌）、真菌和酵母的抗菌活性。结果表明，CS/PAA/Cu-HNCs 的铜释放和溶胀率均高于 CS/Cu-NCs。此外，还研究了在 2016 年和 2017 年两个季节中，使用 CuSO4、CS/Cu-NCs 和 CS/PAA/Cu-HNCs 作为不同铜源对洋葱植物叶绿素含量、营养生长、矿物质含量和产量的影响。研究发现，用负载 75 ppm 铜的 CS/PAA-HNPs 作为洋葱植物的叶面喷雾，可以提高洋葱的产量、植物生长和鳞茎的营养含量。
