一种用于单排卵帚尾袋貂（帚尾袋貂）超数排卵的孕马血清促性腺激素/促性腺激素释放激素方法。

A PMSG/GnRH method for the superovulation of the monovulatory brush-tailed possum (Trichosurus vulpecula).

作者信息

Rodger J C, Mate K E

机构信息

Department of Biological Sciences, University of Newcastle, New South Wales, Australia.

出版信息

J Reprod Fertil. 1988 Jul;83(2):885-91. doi: 10.1530/jrf.0.0830885.

DOI:10.1530/jrf.0.0830885

PMID:3045309

Abstract

Optimal ovarian stimulation with minimal degenerative changes was achieved in brush-tailed possums 3 days after a subcutaneous injection of 10 i.u. PMSG. This treatment alone did not result in ovulation and only rarely in oocyte maturation. Ovulation of 8-24 mature oocytes occurred when on the 3rd day after 10 i.u. PMSG the female received 3 intramuscular injections of 50 micrograms synthetic gonadotrophin-releasing hormone (GnRH) 90 min apart. Superovulation was achieved in immature, cycling, pregnant and lactating females, but not in animals in the preovulatory phase of a natural oestrous cycle.

摘要

在刷尾负鼠皮下注射10国际单位孕马血清促性腺激素（PMSG）3天后，实现了具有最小退化性变化的最佳卵巢刺激。单独这种治疗不会导致排卵，仅极少情况下会出现卵母细胞成熟。当在注射10国际单位PMSG后的第3天，雌性接受3次肌肉注射，每次间隔90分钟，注射50微克合成促性腺激素释放激素（GnRH）时，会排出8 - 24个成熟卵母细胞。超排卵在未成熟、处于发情周期、怀孕和哺乳期的雌性中均能实现，但在自然发情周期的排卵前期动物中无法实现。