Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education, Lab of Biochemistry, College of Chemistry, Xiangtan University, Xiangtan, 411105, China.
Department of Nutrition and Food Hygiene, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, 510515, China.
Eur J Med Chem. 2019 Jan 15;162:423-434. doi: 10.1016/j.ejmech.2018.10.001. Epub 2018 Oct 11.
Multidrug resistance (MDR), defined as the cross-resistance of cancer cells toward a broad range of chemotherapeutic agents, is a universal and intractable problem in chemotherapy. The understanding of MDR mechanisms is essential to discover the potential biomarkers for predicting multidrug resistance and more importantly, tackling and preventing multidrug resistance. Multiple technologies have been used to study MDR mechanisms including comparative genomic hybridization, DNA array, differential display RT-PCR and various immunoassays. Compared with these approaches, proteomic technologies allow a high through-put analysis of protein detection, protein quantification and protein interaction with high accuracy. With the rapid development of proteomic studies in recent years, proteomic technologies have made substantial contributions to the characterization of MDR mechanisms including MDR-related protein detection and quantification, as well as the characterization of drug-transporter binding sites. This review offers a comprehensive illustration of MDR, proteomic technologies and the discoveries made in understanding MDR mechanisms using proteomic approaches.
多药耐药性（MDR）是指癌细胞对多种化疗药物的交叉耐药性，是化疗中的一个普遍且棘手的问题。了解 MDR 机制对于发现潜在的预测多药耐药性的生物标志物至关重要，更重要的是，解决和预防多药耐药性。已经使用多种技术来研究 MDR 机制，包括比较基因组杂交、DNA 芯片、差异显示 RT-PCR 和各种免疫测定。与这些方法相比，蛋白质组学技术允许高通量分析蛋白质检测、蛋白质定量和蛋白质与高准确度的相互作用。近年来，蛋白质组学研究的快速发展为 MDR 机制的特征提供了实质性的贡献，包括 MDR 相关蛋白的检测和定量，以及药物转运体结合位点的特征。本综述全面介绍了 MDR、蛋白质组学技术以及使用蛋白质组学方法在理解 MDR 机制方面的发现。
