在 AOB 和 ANAMMOX 生物量较少的情况下，实现单级脱氨反应器的快速启动和稳定运行。
Quick start-up and stable operation of a one-stage deammonification reactor with a low quantity of AOB and ANAMMOX biomass.
机构信息
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 1239 Siping Road, Shanghai 200092, PR China; Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720, USA.
Department of Civil and Environmental Engineering, University of California, Berkeley, CA 94720, USA; Tsinghua-Berkeley Shenzhen Institute, Tsinghua University Shenzhen, PR China.
出版信息
Sci Total Environ. 2019 Mar 1;654:933-941. doi: 10.1016/j.scitotenv.2018.11.069. Epub 2018 Nov 6.
In this study, a quick start-up of one-stage deammonification in an immobilized aerobic ammonium oxidizing bacteria (AOB) and anoxic ammonium oxidizing (ANAMMOX) bacteria up-flow reactor (IAAR) was successfully achieved. With the aid of gel layers, AOB and ANAMMOX bacteria had excellent spatial distribution, theoretically meeting dissolved oxygen requirements for the simultaneous processes of aerobic and anaerobic ammonium oxidizing. The results indicated that an IAAR containing 0.4 g-VSS L immobilized biomass achieved a nitrogen removal rate (NRR) of 0.53 kg-N m d after only 10 days of operation and subsequently reached a maximum nitrogen removal rate (NRR) of 3.73 kg-N m d. The micro-profiles of DO and pH were measured using microelectrodes to help understand the stratification of the microbial processes inside the gel layers. The distribution of AOB and ANAMMOX bacteria within the gel layers was verified using fluorescence in situ hybridization (FISH) analysis. The community distribution in the FISH three-dimensional images closely corresponded to the micro-profiles of DO concentration and pH, enabling rapid adaptation and stable operation of the reactor seeded with a quite low quantity of biomass.
在这项研究中，成功实现了固定化好氧氨氧化菌（AOB）和缺氧氨氧化菌（ANAMMOX）上流式反应器（IAAR）的单级脱氨快速启动。借助凝胶层，AOB 和 ANAMMOX 细菌具有极好的空间分布，理论上满足好氧和厌氧氨氧化同时进行的溶解氧要求。结果表明，在仅运行 10 天的情况下，含有 0.4 g-VSS/L 固定化生物量的 IAAR 实现了 0.53 kg-N·m-3·d-1 的氮去除率（NRR），随后达到了 3.73 kg-N·m-3·d-1 的最大氮去除率（NRR）。使用微电极测量了 DO 和 pH 的微剖面，以帮助了解凝胶层内微生物过程的分层。使用荧光原位杂交（FISH）分析验证了凝胶层内 AOB 和 ANAMMOX 细菌的分布。FISH 三维图像中的群落分布与 DO 浓度和 pH 的微剖面密切对应，使得在接种相当低数量生物量的情况下，反应器能够快速适应和稳定运行。
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