来自大肠杆菌的腺苷酸琥珀酸合成酶的初步X射线晶体学研究。

Preliminary X-ray crystallographic study of adenylosuccinate synthetase from Escherichia coli.

作者信息

Serra M A, Bass M B, Fromm H J, Honzatko R B

机构信息

Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.

出版信息

J Mol Biol. 1988 Apr 20;200(4):753-4. doi: 10.1016/0022-2836(88)90489-5.

DOI:10.1016/0022-2836(88)90489-5

PMID:3045328

Abstract

Adenylosuccinate synthetase, a dimeric enzyme of 96,000 Mr, catalyzes the first committed step toward the de novo biosynthesis of AMP. Large, single crystals of adenylosuccinate synthetase from Escherichia coli grow from solutions of polyethylene glycol and ammonium sulfate. Crystals from ammonium sulfate belong to the orthorhombic space group P212121 with unit cell parameters a = 79.0 A, b = 70.2 A and c = 152.6 A. Crystals from polyethylene glycol belong to the space group P21, having unit cell parameters of a = 71.16 A, b = 71.99 A, c = 82.95 A, and beta = 71.52 degrees. The asymmetric units of both crystal forms probably contain the entire dimeric enzyme.

摘要

腺苷酸琥珀酸合成酶是一种分子量为96,000的二聚体酶，催化从头合成AMP的第一步关键反应。来自大肠杆菌的腺苷酸琥珀酸合成酶的大单晶在聚乙二醇和硫酸铵溶液中生长。硫酸铵晶体属于正交晶系空间群P212121，晶胞参数为a = 79.0 Å，b = 70.2 Å，c = 152.6 Å。聚乙二醇晶体属于空间群P21，晶胞参数为a = 71.16 Å，b = 71.99 Å，c = 82.95 Å，β = 71.52°。两种晶体形式的不对称单元可能都包含整个二聚体酶。