Shittu Ismaila, Adedeji Adeyinka J, Luka Pam D, Asala Olayinka O, Sati Nancy M, Nwagbo Ijeoma O, Chinyere Chinonyerem N, Arowolo Oluwadamilola O, Adole Jolly A, Emennaa Paulinus, Abdu Paul A, Joannis Tony M
National Veterinary Research Institute, PMB 01, Vom, Nigeria.
National Veterinary Research Institute, PMB 01, Vom, Nigeria.
Biologicals. 2019 Jan;57:29-33. doi: 10.1016/j.biologicals.2018.11.003. Epub 2018 Nov 16.
Globally, vaccines are used to prevent and control the menace of infectious diseases in livestock with some reported to be inadvertently contaminated with extraneous agents (EAs). With the aim of screening and characterizing for some selected EAs, 44 live viral poultry vaccines were randomly selected based on availability. The vaccines comprised 14 manufacturers in 10 different countries including Nigeria were screened by Polymerase Chain Reaction. In 9% (4/44) of the vaccines, contamination with only avian leukosis virus (ALV) subgroup J (ALV-J) was recorded. Other exogenous ALV subgroups, chicken infectious anemia and infectious laryngotracheitis viruses were absent. The EAs was found in infectious bursal disease (n = 1), Fowlpox (n = 2) and Mareks disease (n = 1) vaccines. Phylogenetic analysis of the ALV-J env gene showed clustering with contemporary group I and II. The result underscores the importance of screening vaccines to avoid the introduction and spread of EAs that could pose a threat to poultry production.
在全球范围内，疫苗被用于预防和控制家畜传染病的威胁，但据报道，一些疫苗无意中被外来因子（EA）污染。为了筛选和鉴定某些选定的外来因子，根据可得性随机选择了44种活病毒家禽疫苗。这些疫苗由包括尼日利亚在内的10个不同国家的14家制造商生产，并通过聚合酶链反应进行筛选。在9%（4/44）的疫苗中，仅记录到感染了禽白血病病毒J亚群（ALV-J）。其他外源性禽白血病病毒亚群、鸡传染性贫血病毒和传染性喉气管炎病毒均未检出。在传染性法氏囊病疫苗（n = 1）、鸡痘疫苗（n = 2）和马立克氏病疫苗（n = 1）中发现了外来因子。对ALV-J env基因的系统发育分析表明，其与当代I组和II组聚类。该结果强调了筛选疫苗以避免可能对家禽生产构成威胁的外来因子的引入和传播的重要性。
