Shi Fachao, Yang Xiufen, Zeng Hongmei, Guo Lihua, Qiu Dewen
1Key Laboratory for Biological Control of the Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100081 China.
2Guangdong Engineering Research Center for Pesticide and Fertilizer, Guangdong Provincial Bioengineering Institute (Guangzhou Sugarcane Industry Research Institute), Guangzhou, 510316 China.
3 Biotech. 2018 Nov;8(11):480. doi: 10.1007/s13205-018-1507-5. Epub 2018 Nov 13.
The protein elicitor PeaT1 was found in and exerted broad spectrum resistance in wheat, cotton, and rice. Recently, we found that overexpressing rice (OE) could enhance plant drought tolerance. Elucidating some elevated drought stress-related proteins and associated mechanisms is inevitable for improving drought tolerance in rice. In this study, combining a label-free quantitative proteomic method, multiple proteins were differentially accumulated in OE plants. Among these, a total of 57 significant changed proteins (including 32 up-regulated and 25 down-regulated) were mainly involved in metabolic, cellular, biological progress, and stress response. Using the RT-qPCR assay, 18 proteins' relative abundance was detected mostly consistent with the proteins abundance in proteomic data. Specially, proteins involved in abiotic stress, such as and , which were significantly induced in early after dehydration treatment in transgenic rice, and the other stress response genes (prohibitin protein, PsbP protein, msrB Protein) also changed in PeaT1 OE lines. Taken together, these results suggested that these differential proteins would be helpful for understanding the functional molecular mechanism of PeaT1 in rice.
蛋白激发子PeaT1在小麦、棉花和水稻中被发现并具有广谱抗性。最近，我们发现过表达水稻（OE）可以增强植物的耐旱性。阐明一些与干旱胁迫相关的上调蛋白及其相关机制对于提高水稻的耐旱性是必不可少的。在本研究中，结合无标记定量蛋白质组学方法，在OE植株中有多种蛋白质差异积累。其中，共有57种显著变化的蛋白质（包括32种上调和25种下调）主要参与代谢、细胞、生物学进程和应激反应。使用RT-qPCR分析，检测到18种蛋白质的相对丰度大多与蛋白质组学数据中的蛋白质丰度一致。特别地，参与非生物胁迫的蛋白质，如在转基因水稻脱水处理早期显著诱导的[具体蛋白质名称缺失]和[具体蛋白质名称缺失]，以及其他应激反应基因（抗增殖蛋白、光系统II外周蛋白、蛋氨酸亚砜还原酶B蛋白）在PeaT1 OE系中也发生了变化。综上所述，这些结果表明这些差异蛋白质将有助于理解PeaT1在水稻中的功能分子机制。
