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用于表征一种在切尔诺贝利核电站附近分离出的耐铀放线菌的蛋白质组学数据。

Proteomics data for characterizing , an uranium-tolerant actinobacterium isolated near the Chernobyl nuclear power plant.

作者信息

Gallois Nicolas, Piette Laurie, Ortet Philippe, Barakat Mohamed, Long Justine, Berthomieu Catherine, Armengaud Jean, Chapon Virginie, Alpha-Bazin Béatrice

机构信息

CEA, CNRS, Aix-Marseille Université, UMR 7265 Biologie Végétale et Microbiologie Environnementales, Laboratoire des Interactions Protéine Métal, 13108 Saint-Paul-lez-Durance, France.

CEA, CNRS, Aix-Marseille Université, UMR 7265 Biologie Végétale et Microbiologie Environnementales, Laboratoire d׳écologie microbienne de la rhizosphère et d׳environnements extrêmes, 13108 Saint-Paul-lez-Durance, France.

出版信息

Data Brief. 2018 Oct 30;21:1125-1129. doi: 10.1016/j.dib.2018.10.136. eCollection 2018 Dec.

Abstract

A9 cells were exposed or not to 10 µM uranyl nitrate as resting cells in sodium chloride solution. Bacteria exposed to U(VI) and controls were harvested after 0.5, 4, and 24 h of toxicant exposure. Bacteria were subjected to high-throughput proteomics analysis using a Q-Exactive HF high resolution tandem mass spectrometer incorporating an ultra-high-field orbitrap analyzer. MS/MS spectra were assigned with a protein sequence database derived from a draft genome obtained by Illumina sequencing and systematic six-reading frame translation of all the contigs. Proteins identified in bacteria exposed to U(VI) and controls at the three time points allow defining the proteome dynamics upon uranium stress. The data reported here are related to a published study regarding the proteome dynamics of A9 upon uranium stress by Gallois et al. (in press) entitled "Proteogenomic insights into uranium tolerance of a Chernobyl׳s Microbacterium bacterial isolate". The data accompanying the manuscript describing the database searches and comparative analysis have been deposited to the ProteomeXchange with identifier PXD005794.

摘要

将A9细胞作为静息细胞置于氯化钠溶液中,使其暴露或不暴露于10µM硝酸铀酰。暴露于U(VI)的细菌和对照在接触毒物0.5、4和24小时后收获。使用配备超高场轨道阱分析仪的Q-Exactive HF高分辨率串联质谱仪对细菌进行高通量蛋白质组学分析。MS/MS谱图通过一个蛋白质序列数据库进行比对,该数据库来源于通过Illumina测序获得的基因组草图以及对所有重叠群进行的系统六读框翻译。在三个时间点暴露于U(VI)的细菌和对照中鉴定出的蛋白质,有助于确定铀胁迫下的蛋白质组动态变化。此处报告的数据与Gallois等人(即将发表)的一项已发表研究相关,该研究题为“切尔诺贝利微杆菌分离株对铀耐受性的蛋白质基因组学见解”,涉及A9在铀胁迫下的蛋白质组动态变化。描述数据库搜索和比较分析的手稿所附数据已存入蛋白质组交换库,标识符为PXD005794。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/29ff/6231083/52f28a5c8077/gr1.jpg

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