Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton, Victoria 3800, Australia.
Analyst. 2018 Dec 3;143(24):6087-6094. doi: 10.1039/c8an01558a.
Attenuated Total Reflection Fourier Transform Infrared (ATR-FT-IR) spectroscopy has been applied to compare the effect of the new organoamidoplatinum(ii) complexes [Pt{NH(p-HC6F4)CH2CH2N(p-HC6F4)}(py)(O2CR)] (R = C6F4 or 2,4,6-Me3C6H2) with cisplatin on cells from one cisplatin-sensitive ovarian cancer cell line (A2780) and one cisplatin-resistant ovarian cancer cell line (A2780R). After incubation of the cells with cisplatin, 1 and 2 for 48 hours, distinct changes were found in the ATR-FT-IR spectra. Comparison of the second derivative spectra suggests that 1 and 2 induce similar chemical changes in both cell lines, A2780 and A2780R, while cisplatin had a slight effect on A2780 and A2780R cells. Furthermore, drugs 1 and 2 result in changes to the phosphodiester and polysaccharide bands in the spectra. This work shows how ATR-FT-IR can be applied to monitor the effects of organoamidoplatinum(ii) complexes on cisplatin-sensitive and cisplatin-resistant cell lines providing potential information on how drugs affect the cellular metabolism.
衰减全反射傅里叶变换红外（ATR-FT-IR）光谱已被应用于比较新型有机酰胺铂（II）配合物[Pt{NH（p-HC6F4）CH2CH2N（p-HC6F4）}（py）（O2CR）]（R = C6F4 或 2,4,6-Me3C6H2）与顺铂对来自一种顺铂敏感卵巢癌细胞系（A2780）和一种顺铂耐药卵巢癌细胞系（A2780R）的细胞的影响。细胞与顺铂、1 和 2孵育 48 小时后，ATR-FT-IR 光谱中发现了明显的变化。二阶导数光谱的比较表明，1 和 2 在两种细胞系 A2780 和 A2780R 中引起相似的化学变化，而顺铂对 A2780 和 A2780R 细胞的影响较小。此外，药物 1 和 2 导致光谱中磷酸二酯和多糖带的变化。这项工作展示了 ATR-FT-IR 如何应用于监测有机酰胺铂（II）配合物对顺铂敏感和耐药细胞系的影响，为药物如何影响细胞代谢提供了潜在信息。
