pYAC-RC, a yeast artificial chromosome vector for cloning DNA cut with infrequently cutting restriction endonucleases.
作者信息
Marchuk D, Collins F S
机构信息
Department of Human Genetics, University of Michigan Medical Center, Ann Arbor 48109-0650.
出版信息
Nucleic Acids Res. 1988 Aug 11;16(15):7743. doi: 10.1093/nar/16.15.7743.
Abstract
摘要
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pYAC-RC, a yeast artificial chromosome vector for cloning DNA cut with infrequently cutting restriction endonucleases.pYAC - RC，一种用于克隆由稀有切割限制性内切酶切割的DNA的酵母人工染色体载体。
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Isolation of telomeric DNA from the filamentous fungus Podospora anserina and construction of a self-replicating linear plasmid showing high transformation frequency.从丝状真菌嗜热栖热放线菌中分离端粒DNA并构建具有高转化频率的自我复制线性质粒。
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本文引用的文献
1
Cloning of large segments of exogenous DNA into yeast by means of artificial chromosome vectors.通过人工染色体载体将大片段外源DNA克隆到酵母中。
Science. 1987 May 15;236(4803):806-12. doi: 10.1126/science.3033825.
2
Strategies for mapping and cloning macroregions of mammalian genomes.哺乳动物基因组大区域的定位与克隆策略。
Methods Enzymol. 1987;151:461-89. doi: 10.1016/s0076-6879(87)51038-2.
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