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一系列简化哺乳动物基因靶向的载体。

A series of vectors that simplify mammalian gene targeting.

作者信息

Brookes A J, Stevenson B J, Porteous D J, Dorin J R

机构信息

MRC Human Genetics Unit, Western General Hospital, Edinburgh, UK.

出版信息

Transgenic Res. 1993 Jul;2(4):238-44. doi: 10.1007/BF01977354.

DOI:10.1007/BF01977354
PMID:8364606
Abstract

In order to facilitate the procedure of mammalian gene targeting, we have produced and functionally tested a series of generic vectors. Homologous recombination has been achieved with each vector. The vectors are designed for both replacement and insertional recombination, are suitable for 'hit and run' strategies and contain all necessary genetic elements for both positive-negative and promoterless/gene fusion enrichment of homologous integrations. Multiple unique restriction sites are included to simplify the incorporation of genomic targeting sequences.

摘要

为了促进哺乳动物基因打靶过程，我们构建并对一系列通用载体进行了功能测试。每个载体都实现了同源重组。这些载体设计用于置换和插入重组，适用于“打了就跑”策略，并且包含用于同源整合的正负筛选和无启动子/基因融合富集的所有必要遗传元件。载体中包含多个独特的限制性酶切位点，以简化基因组靶向序列的掺入。

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A zinc finger truncation of murine WT1 results in the characteristic urogenital abnormalities of Denys-Drash syndrome.小鼠WT1的锌指截短导致Denys-Drash综合征的特征性泌尿生殖系统异常。
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Establishment in culture of pluripotential cells from mouse embryos.从小鼠胚胎中建立多能细胞的培养方法。
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.脂质体转染法：一种高效的、脂质介导的DNA转染程序。
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