Guo Liang, Zhang Chao, Chen Guowei, Wu Man, Liu Wukang, Ding Chengchao, Dong Qingli, Fan Enguo, Liu Qing
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China.
School of Medical Instrument and Food Engineering, University of Shanghai for Science and Technology, Shanghai, 200093, China; Institute of Biochemistry and Molecular Biology, ZBMZ, University of Freiburg, Freiburg, 79104, Germany.
Microb Pathog. 2019 Feb;127:183-189. doi: 10.1016/j.micpath.2018.11.023. Epub 2018 Nov 17.
Although the level of reactive oxygen species (ROS) is altered upon the formation of bacterial biofilm, the relationship between ROS alteration and biofilm formation is still unclear. The aim of the present study is to use Listeria monocytogenes (L. monocytogenes) as a model organism to examine whether ROS have an effect on the biofilm formation. After eliminating ROS by treatment with NAD(P)H oxidase inhibitor Diphenyleneiodonium chloride (DPI) or scavenging reagents N-acetylcysteine (NAC), the biofilm formation of L. monocytogenes was examined. Our data demonstrate that DPI and NAC induced-reduction of ROS enhances the biofilm formation in L. monocytogenes without affecting bacterial growth and activity. These data provide the evidence that ROS produced by L. monocytogenes inhibit the biofilm formation.
尽管在细菌生物膜形成时活性氧（ROS）水平会发生改变，但ROS改变与生物膜形成之间的关系仍不清楚。本研究的目的是以单核细胞增生李斯特菌作为模式生物，来检测ROS是否对生物膜形成有影响。在用NAD（P）H氧化酶抑制剂二苯基碘鎓氯化物（DPI）处理或用清除剂N-乙酰半胱氨酸（NAC）清除ROS后，检测了单核细胞增生李斯特菌的生物膜形成情况。我们的数据表明，DPI和NAC诱导的ROS减少增强了单核细胞增生李斯特菌的生物膜形成，而不影响细菌的生长和活性。这些数据提供了证据，证明单核细胞增生李斯特菌产生的ROS会抑制生物膜形成。
