1Department of Stomatology, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, Nanchong, Sichuan 637000 People's Republic of China.
2Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, The Second Clinical Medical College of North Sichuan Medical College, No. 95, People's south Road, Shunqing District, Nanchong, Sichuan 637000 People's Republic of China.
Cell Mol Biol Lett. 2018 Nov 15;23:53. doi: 10.1186/s11658-018-0120-2. eCollection 2018.
Oral cancer remains a deadly disease worldwide. Lymph node metastasis and invasion is one of the causes of death from oral cancer. Elucidating the mechanism of oral cancer lymph node metastasis and identifying critical regulatory genes are important for the treatment of this disease. This study aimed to identify differentially expressed genes (gene signature) and pathways that contribute to oral cancer metastasis to lymph nodes. The GSE70604-associated study compared gene profiles in lymph nodes with metastasis of oral cancer to those of normal lymph nodes. The GSE2280-associated study compared gene profiles in primary tumor of oral cancer with lymph node metastasis to those in tumors without lymph node metastasis. There are 28 common differentially expressed genes (DEGs) showing consistent changes in both datasets in overlapping analysis. GO biological process and KEGG pathway analysis of these 28 DEGs identified the gene signature CCND1, JUN and SPP1, which are categorized as key regulatory genes involved in the focal adhesion pathway. Silencing expression of CCND1, JUN and SPP1 in the human oral cancer cell line OECM-1 confirmed that those genes play essential roles in oral cancer cell invasion. Analysis of clinical samples of oral cancer found a strong correlation of these genes with short survival, especially JUN expression associated with metastasis. Our study identified a unique gene signature - CCND1, JUN and SPP1 - which may be involved in oral cancer lymph node metastasis.
口腔癌仍然是一种全球性的致命疾病。淋巴结转移和侵袭是口腔癌死亡的原因之一。阐明口腔癌淋巴结转移的机制并确定关键调控基因对于治疗这种疾病非常重要。本研究旨在鉴定与口腔癌淋巴结转移相关的差异表达基因（基因特征）和途径。GSE70604 相关研究比较了口腔癌淋巴结转移和正常淋巴结的基因图谱。GSE2280 相关研究比较了口腔癌原发肿瘤有淋巴结转移和无淋巴结转移的基因图谱。在重叠分析中，有 28 个共同的差异表达基因（DEGs）在两个数据集均显示出一致的变化。这些 28 个 DEGs 的 GO 生物过程和 KEGG 通路分析确定了基因特征 CCND1、JUN 和 SPP1，它们被归类为参与粘着斑途径的关键调控基因。在人口腔癌细胞系 OECM-1 中沉默 CCND1、JUN 和 SPP1 的表达证实了这些基因在口腔癌细胞侵袭中发挥着重要作用。对口腔癌临床样本的分析发现，这些基因与短生存期有很强的相关性，尤其是 JUN 表达与转移相关。我们的研究确定了一个独特的基因特征——CCND1、JUN 和 SPP1，它们可能参与了口腔癌的淋巴结转移。
