Hosseini Seyed Mojtaba, Sharafkhah Ali, Ziaee Seyyed Mohyeddin
Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran.
Cell and Molecular Medicine Student Research Group, Medical Faculty, Shiraz University of Medical Sciences, Shiraz, Iran.
Asian J Neurosurg. 2018 Oct-Dec;13(4):1101-1107. doi: 10.4103/ajns.AJNS_140_17.
Spinal cord injury (SCI) as one of the most important diseases of central nervous system (CNS) without any definite treatment is still growing in incidence. In addition to trauma, some surgeries such as cardiac and thoracic aorta surgery may result in SCI as a complication. In last years, a promising approach has shed light on this CNS injury thanks to stem cell technology. Stem cell therapy could be considered as a good candidate for transplantation and enhancing neural regeneration in SCI. In this study, we identified the effects of spinal cord-derived neural precursor cells (NPCs) transplantation on SCI in after and before injury injection.
NPCs were isolated from the adult rat spinal cord and cultured using complete culture media. After neurosphere formation, the cells were differentiated to neurons, oligodendrocytes, and astrocyte. The cells were transplanted to the rat model of SCI in 1 day before and 1 day after injury. The animals were followed for 12 weeks to assess their neurological performance. In addition, histological study and inflammatory cytokines levels have been studied.
Our results indicate that NPCs infusion both pre- and post-SCI could decrease the level of inflammatory cytokines. In addition, the neurological performance and histologic studies showed recovery after this type of injury using NPCs, and it might be due to inflammation modulatory effects on neural stem cells.
NPCs therapy for SCI in both two-time points (before and after SCI) could be beneficial and make a neurological recovery. In other words, NPCs therapy could be considered as a therapeutic and also preventive approach for SCI.
脊髓损伤（SCI）作为中枢神经系统（CNS）最重要的疾病之一，尚无确切治疗方法，其发病率仍在上升。除创伤外，一些手术如心脏和胸主动脉手术也可能导致SCI作为并发症。近年来，由于干细胞技术，一种有前景的方法为这种中枢神经系统损伤带来了曙光。干细胞疗法可被视为脊髓损伤移植和促进神经再生的良好候选方法。在本研究中，我们确定了脊髓源性神经前体细胞（NPCs）移植在损伤前后注射对脊髓损伤的影响。
从成年大鼠脊髓中分离NPCs，并用完全培养基培养。神经球形成后，将细胞分化为神经元、少突胶质细胞和星形胶质细胞。在损伤前1天和损伤后1天将细胞移植到脊髓损伤大鼠模型中。对动物进行12周的随访以评估其神经功能。此外，还进行了组织学研究和炎症细胞因子水平研究。
我们的结果表明，脊髓损伤前后注入NPCs均可降低炎症细胞因子水平。此外，神经功能和组织学研究表明，使用NPCs可使这种类型的损伤后恢复，这可能是由于对神经干细胞的炎症调节作用。
在两个时间点（脊髓损伤前后）对脊髓损伤进行NPCs治疗可能是有益的，并可实现神经功能恢复。换句话说，NPCs治疗可被视为脊髓损伤的治疗和预防方法。
