Li Qian, Zhang Shuang, Zheng Yanjie, Wen Hebao, Han Xiao, Zhang Minghai, Guan Weijun
College of Wildlife Resources, Northeast Forestry University, Harbin, PR China.
Scientific Experiment Research Center, Harbin Institute of Physical Education, Harbin, PR China.
Anim Cells Syst (Seoul). 2017 Aug 3;21(4):233-240. doi: 10.1080/19768354.2017.1354915. eCollection 2017.
Neural stem cells (NSCs) are multipotent stem cells that can differentiate into many cell types in vitro. In this study, we isolated and established an NSC line from fetal . Based on the results of immunofluorescence staining, NSCs expressed Nestin, Pax6 and MAP2. Moreover, a reverse transcription-polymerase chain reaction assay was used to biologically characterize the cell line. NSCs were induced to differentiate into neurogenic cells in vitro. They expressed MAP2, glial fibrillary acidic protein (GFAP) and myelin basic protein (MBP). In this study, we successfully isolated and cultivated NSCs from the hippocampal tissue of fetal sheep. NSCs not only displayed a self-renewal capacity but also had the potential to differentiate into neurons and glial cells. This study provided valuable experimental data for NSC transplant research.
神经干细胞（NSCs）是多能干细胞，能够在体外分化为多种细胞类型。在本研究中，我们从胎儿[具体来源未给出]分离并建立了一个神经干细胞系。基于免疫荧光染色结果，神经干细胞表达巢蛋白、Pax6和微管相关蛋白2（MAP2）。此外，采用逆转录-聚合酶链反应分析对该细胞系进行生物学特性鉴定。神经干细胞在体外被诱导分化为神经源性细胞。它们表达MAP2、胶质纤维酸性蛋白（GFAP）和髓鞘碱性蛋白（MBP）。在本研究中，我们成功地从胎羊海马组织中分离并培养了神经干细胞。神经干细胞不仅表现出自我更新能力，还具有分化为神经元和神经胶质细胞的潜力。本研究为神经干细胞移植研究提供了有价值的实验数据。
