Jang Sang-Pil, Park Seong-Hwan, Jung Jun-Sub, Lee Hee-Jung, Hong Jung-Woo, Lee Jae-Yong, Suh Hong-Won
Department of Pharmacology, Institute of Natural Medicine, College of Medicine, Hallym University, Chuncheon, Korea.
Department of Life science, Hallym University, Chuncheon, Korea.
Anim Cells Syst (Seoul). 2018 Jun 28;22(4):227-233. doi: 10.1080/19768354.2018.1490348. eCollection 2018.
Fasting in general causes several metabolic changes. In the present study, we examined the possible changes of several types of nociception during the food deprivation were investigated in mice. After the mice were forced into the fasting for 12, 24, or 48 h, the changes of nociception were measured by the tail-flick, writhing, formalin or von-frey tests. We found that the nociceptive behavior induced by intraperitoneally (i.p.) administered acetic acid (writhing response) or intraplantar injection of 5% formalin into the hind-paw were reduced in fasted group. In addition, the tail-flick response and threshold for nociception in mechanical von-frey test were also elevated in fasted group. Moreover, the p-CREB and p-ERK levels in the dorsal root ganglia (DRG) and the spinal cord were reduced in food-deprived group. Furthermore, p-AMPKα expressions in DRG and the spinal cord were up-regulated, whereas p-mTOR in DRG and the spinal cord was down-regulated in food-deprived group. Our results suggest that the chemical, mechanical, and thermal nociceptions appear to be reduced in a food-deprived mouse group. Additionally, reduction of nociception in food-deprived group appears to be closely associated with the expressions of several signal transduction molecules such as ERK, CREB, AMPKα and mTOR proteins in DRG and the spinal cord.
一般来说，禁食会引起多种代谢变化。在本研究中，我们检测了小鼠在食物剥夺期间几种伤害感受的可能变化。将小鼠强制禁食12、24或48小时后，通过甩尾、扭体、福尔马林或von-frey试验测量伤害感受的变化。我们发现，禁食组中腹腔注射（i.p.）醋酸（扭体反应）或后爪足底注射5%福尔马林诱导的伤害感受行为减少。此外，禁食组在机械性von-frey试验中的甩尾反应和伤害感受阈值也升高。此外，食物剥夺组背根神经节（DRG）和脊髓中的p-CREB和p-ERK水平降低。此外，食物剥夺组DRG和脊髓中的p-AMPKα表达上调，而DRG和脊髓中的p-mTOR下调。我们的结果表明，食物剥夺小鼠组的化学、机械和热伤害感受似乎降低。此外，食物剥夺组伤害感受的降低似乎与DRG和脊髓中几种信号转导分子如ERK、CREB、AMPKα和mTOR蛋白的表达密切相关。
