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[一种简单的高灵敏度记录式显微分光光度计]

[A simple highly sensitive recording microspectrophotometer].

作者信息

Govardovskiĭ V I, Zueva L V

出版信息

Tsitologiia. 1988 Apr;30(4):499-502.

PMID:3046083
Abstract

A design of the recording microspectrophotometer is described. The instrument possesses an absolutely flat base line and quantum-noise limited detection threshold. Two principal elements of the design are the "jumping" stage, and the logarithmic amplifier with the phase-sensitive detector which converts the photomultiplier output into the optical density signal. The performance of the instrument is illustrated by the recordings of visual pigment spectra in single photoreceptors.

摘要

本文描述了一种记录式显微分光光度计的设计。该仪器具有绝对平坦的基线和量子噪声限制的检测阈值。该设计的两个主要元件是“跳跃”平台和带有相敏检测器的对数放大器,相敏检测器将光电倍增管的输出转换为光密度信号。通过记录单个光感受器中的视觉色素光谱来说明该仪器的性能。

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