Altaş Bengül, Koçak Hasan Emre, Altınay Serdar, Yücebaş Kadir, Taşkın Ümit, Oktay Mehmet Faruk
BağcılarTraining and Research Hospital, Department of Otorhinolaryngology - Head and Neck Surgery, Istanbul, Turkey.
Bakırköy Dr.Sadi Konuk Training and Research Hospital, Department of Otorhinolaryngology - Head and Neck Surgery, Istanbul, Turkey.
Otolaryngol Pol. 2018 Jun 20;72(5):37-44. doi: 10.5604/01.3001.0012.1265.
Atrophic rhinitis (AR) is a disease characterized by atrophy of the mucosa, submucosa, bone tissue due to an unknown cause and excessive nasal cavity enlargement. The disease still has no complete treatment, and the treatment of the functional loss of atrophic cells in AR is still a matter to be investigated. Ozone (O3) therapy has been shown to enhance cell metabolism, angiogenesis, fibroblast activity, and collagen synthesis.
To determine whether ozone treatment affects the disease histopathologically, in experimentally created AR. Material - Method: Twelve Wistar Hanover strain albino male rats were included in the study. Atrophic rhinitis was induced in animals by administering Pasteurella multocida toxin diluted with saline for 21 days to both nasal cavities. A total of 12 animals included in the study were divided into 2 groups as control and study. Ozone gas (60 μg/mL) was administered rectally to the study group for 21 days. After 2 weeks, the rats were decapitated, the nasal cavities were removed as a block, and atrophic rhinitis parameters (epithelial hyperplasia, goblet cell loss, cilia loss, inflammatory infiltration, and vascular ectasia) were evaluated under light microscopy by histopathological examination and statistically interpreted.
The incidence of vascular ectasia was significantly lower in the ozone group compared to the control group (p<0.05). There was no significant difference between the groups regarding other histopathologic findings.
Ozone treatment was moderate at the histopathological level. We concluded that ozone therapy has no or very limited effect on atrophic rhinitis.
萎缩性鼻炎（AR）是一种由于不明原因导致黏膜、黏膜下层和骨组织萎缩以及鼻腔过度扩大的疾病。该疾病尚无彻底的治疗方法，AR中萎缩细胞功能丧失的治疗仍有待研究。臭氧（O3）疗法已被证明可增强细胞代谢、血管生成、成纤维细胞活性和胶原蛋白合成。
确定在实验性诱导的AR中，臭氧治疗是否会对疾病产生组织病理学影响。材料与方法：本研究纳入12只Wistar Hanover品系白化雄性大鼠。通过向双侧鼻腔注射用生理盐水稀释的多杀巴斯德菌毒素21天，诱导动物发生萎缩性鼻炎。本研究纳入的12只动物分为对照组和研究组。研究组经直肠给予臭氧气体（60μg/mL），持续21天。2周后，将大鼠断头，整块取出鼻腔，通过组织病理学检查在光学显微镜下评估萎缩性鼻炎参数（上皮增生、杯状细胞缺失、纤毛缺失、炎症浸润和血管扩张），并进行统计学分析。
与对照组相比，臭氧组血管扩张的发生率显著降低（p<0.05）。两组在其他组织病理学发现方面无显著差异。
臭氧治疗在组织病理学水平上效果中等。我们得出结论，臭氧疗法对萎缩性鼻炎没有或只有非常有限的效果。
