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鉴定、分类和功能分析沙漠苔藓中的家族基因。

Identification, Classification, and Functional Analysis of Family Genes in the Desert Moss .

机构信息

Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China.

School of Life Sciences and State Key Laboratory of Agrobiotechnology, The Chinese University of Hong Kong, Hong Kong, China.

出版信息

Int J Mol Sci. 2018 Nov 19;19(11):3637. doi: 10.3390/ijms19113637.

Abstract

is a desert moss which shows tolerance to the desert environment and is emerging as a good plant material for identification of stress-related genes. transcription factor family plays important roles in plant responses to biotic and abiotic stresses. genes have been identified and extensively studied in many plants, while they are rarely studied in moss. In the present study, we identified 83 genes based on the comprehensive dehydrationrehydration transcriptomic atlas of . genes can be classified into five families, including 11 AP2s, 43 DREBs, 26 ERFs, 1 RAV, and 2 Soloists. RNA-seq data showed that 83 exhibited elevated transcript abundances during dehydration⁻rehydration process. We used RT-qPCR to validate the expression profiles of 12 representative and confirmed the expression trends using RNA-seq data. Eight out of 12 BaAP2/ERFs demonstrated transactivation activities. Seven BaAP2/ERFs enhanced salt and osmotic stress tolerances of yeast. This is the first study to provide detailed information on the identification, classification, and functional analysis of the in . This study will lay the foundation for the further functional analysis of these genes in plants, as well as provide greater insights into the molecular mechanisms of abiotic stress tolerance of .

摘要

是一种荒漠苔藓,对荒漠环境具有耐受性,正在成为鉴定与应激相关基因的良好植物材料。 转录因子家族在植物响应生物和非生物胁迫中发挥重要作用。在许多植物中已经鉴定和广泛研究了 基因,但在苔藓中很少研究。在本研究中,我们根据 的全面脱水-复水转录组图谱鉴定了 83 个 基因。这些基因可以分为五个家族,包括 11 个 AP2s、43 个 DREBs、26 个 ERFs、1 个 RAV 和 2 个 Soloists。RNA-seq 数据显示,在脱水-复水过程中,83 个 基因的转录丰度升高。我们使用 RT-qPCR 验证了 12 个代表性 基因的表达谱,并使用 RNA-seq 数据验证了它们的表达趋势。12 个 中的 8 个具有转录激活活性。7 个 BaAP2/ERFs 增强了酵母的耐盐和耐渗透胁迫能力。这是首次在 中对 基因进行鉴定、分类和功能分析的详细研究。本研究将为这些基因在植物中的进一步功能分析奠定基础,并为 耐非生物胁迫的分子机制提供更深入的了解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6fd2/6275083/373bffc96d6b/ijms-19-03637-g001.jpg

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