College of Agronomy, National Key Laboratory of Wheat and Maize Crop Science, Henan Agricultural University, Zhengzhou, 450002, China.
Department of Biological Sciences, Michigan Technological University, Houghton, MI, 49931, USA.
BMC Plant Biol. 2018 Nov 21;18(1):290. doi: 10.1186/s12870-018-1460-9.
Photoperiod-sensitivity is a critical endogenous regulatory mechanism for plant growth and development under specific environmental conditions, while phosphate and sucrose signaling processes play key roles in cell growth and organ initiation. MicroRNA399 is phosphate-responsive, but, whether it has roles in other metabolic processes remains unknown.
MicroRNA399 was determined to be sucrose-responsive through a microRNA array assay. High levels of sucrose inhibited the accumulation of microRNA399 family under phosphate starvation conditions in Arabidopsis thaliana. Similarly, exogenous sucrose supplementation also reduced microRNA399 expression in maize at developmental transition stages. RNA sequencing of a near-isogenic line(photoperiod-sensitive) line and its recurrent parent Huangzao4, a photoperiod-insensitive line, was conducted at various developmental stages. Members of microRNA399 family were down-regulated under long-day conditions in the photoperiod-sensitive near-isogenic line that accumulated more sucrose in vivo compared with the control line Huangzao4.
MicroRNA399s may play central roles in the integration of sucrose sensing and photoperiodic responses under long day conditions in maize.
光周期敏感性是植物在特定环境条件下生长和发育的关键内源性调节机制，而磷酸盐和蔗糖信号转导过程在细胞生长和器官起始中发挥关键作用。microRNA399 对磷酸盐有响应，但它是否在其他代谢过程中发挥作用尚不清楚。
通过 microRNA 阵列分析确定 microRNA399 对蔗糖有响应。在拟南芥中，高浓度的蔗糖在磷酸盐饥饿条件下抑制 microRNA399 家族的积累。同样，外源蔗糖补充也降低了玉米在发育过渡阶段的 microRNA399 表达。在不同发育阶段对近等基因系（光周期敏感）及其轮回亲本黄早 4 号（光周期不敏感）进行 RNA 测序。在光周期敏感的近等基因系中，microRNA399 家族成员在长日照条件下下调，与对照系黄早 4 号相比，该系体内积累了更多的蔗糖。
microRNA399s 可能在玉米在长日照条件下蔗糖感应和光周期响应的整合中发挥核心作用。
