Parodi Monica, Meazza Raffaella, Vitale Chiara, Pietra Gabriella, Carrega Paolo, Vitale Massimo
UOC Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.
Department of Experimental Medicine (DIMES), University of Genova, Genoa, Italy.
Methods Mol Biol. 2019;1884:87-117. doi: 10.1007/978-1-4939-8885-3_6.
This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34 precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.
本章将描述从外周血（PB）或组织中分离和扩增自然杀伤（NK）细胞用于“体外”研究的当前方法，包括NK细胞的抗肿瘤免疫功能。此外，还将描述从CD34前体细胞诱导NK细胞成熟、分化和扩增的方法。最后，还将探讨新的功能和表型定义的NK细胞亚群特征这一热点问题。
