自然杀伤细胞及其前体的分离、扩增与特性分析：作为研究癌症免疫监视的工具

Isolation, Expansion, and Characterization of Natural Killer Cells and Their Precursors as a Tool to Study Cancer Immunosurveillance.

作者信息

Parodi Monica, Meazza Raffaella, Vitale Chiara, Pietra Gabriella, Carrega Paolo, Vitale Massimo

机构信息

UOC Immunologia, IRCCS Ospedale Policlinico San Martino, Genova, Italy.

Department of Experimental Medicine (DIMES), University of Genova, Genoa, Italy.

出版信息

Methods Mol Biol. 2019;1884:87-117. doi: 10.1007/978-1-4939-8885-3_6.

DOI:10.1007/978-1-4939-8885-3_6

PMID:30465197

Abstract

This chapter will describe the current methodologies to isolate and expand NK cells from Peripheral Blood (PB) or tissues for "in vitro" studies, including NK cell antitumor immune function. In addition, methods to induce NK cell maturation, differentiation, and expansion from CD34 precursors will also be described. Finally, it will also be treated the topical issue of the characterization of new functionally and phenotypically defined NK cell subsets.

摘要

本章将描述从外周血（PB）或组织中分离和扩增自然杀伤（NK）细胞用于“体外”研究的当前方法，包括NK细胞的抗肿瘤免疫功能。此外，还将描述从CD34前体细胞诱导NK细胞成熟、分化和扩增的方法。最后，还将探讨新的功能和表型定义的NK细胞亚群特征这一热点问题。