Pan M R, Qiang H S, Shen B H, Yan H, Xiang P
Shenyang Pharmaceutical University, Shenyang 110016, China.
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Science, Shanghai 200063, China.
Fa Yi Xue Za Zhi. 2018 Aug;34(4):375-378. doi: 10.12116/j.issn.1004-5619.2018.04.006. Epub 2018 Aug 25.
To establish a rapid determination method with LC-MS/MS for cocaine and its metabolite benzoylecgonine in hair.
Deuterated internal standards （cocaine-D₃ and benzoylecgonine-D₈） were added to the decontaminated hair. After the extraction by ultrasonication with methanol, the compounds were separated by the Restek Allure PFP propyl column, and cocaine and benzoylecgonine were simultaneously analysed in multiple reaction monitoring mode.
The cocaine and benzoylecgonine in hair showed a good linearity in the range of mass fraction between 0.02 and 10.00 ng/mg with the limits of detection of 0.01 ng/mg.
The developed method is simple and rapid with a good selectivity, which is suitable for the determination of cocaine and its metabolite benzoylecgonine in hair.
建立一种用液相色谱-串联质谱法快速测定毛发中可卡因及其代谢物苯甲酰爱康宁的方法。
将氘代内标（可卡因-D₃和苯甲酰爱康宁-D₈）加入到经过去污处理的毛发中。用甲醇超声提取后，化合物通过Restek Allure PFP丙基柱进行分离，并在多反应监测模式下同时分析可卡因和苯甲酰爱康宁。
毛发中的可卡因和苯甲酰爱康宁在质量分数0.02至10.00 ng/mg范围内呈现良好的线性关系，检测限为0.01 ng/mg。
所建立的方法简便、快速，选择性良好，适用于毛发中可卡因及其代谢物苯甲酰爱康宁的测定。
