Faculty of Medicine, Tokat Gaziosmanpasa University, Department of Medical Biology, Tokat, Turkey.
Faculty of Medicine, Tokat Gaziosmanpasa University, Department of Medical Biology, Tokat, Turkey.
Gene. 2019 Mar 1;687:188-192. doi: 10.1016/j.gene.2018.11.059. Epub 2018 Nov 19.
Crimean-Congo hemorrhagic fever (CCHF) is an acute viral hemorrhagic fever caused by Crimean Congo hemorrhagic fever virus (CCHFV). Paraoxonase-1 (PON1) is a high density lipoprotein (HDL)-binding protein which defense the body against oxidative stress. To investigate the role of the PON1 gene in CCHF, we screened the genotypes of two single nucleotide polymorphisms (Q192R [rs662] and L55M [rs854560]) in CCHF patients stratified according to course of severity and mortality by using PCR-based RFLP assay. Overall, 132 patients diagnosed as CCHF were enrolled in this study. The frequencies of the three genotypes and two alleles of Q192R and L55M polymorphisms didn't show any statistically significant differences in terms of mortality and disease severity (p > 0.05). Any statistically significant differences were not found between severe and mild and fatal and non-fatal CCHF patients according to seven composite genotypes (p > 0.05). When we analyzed the clinical characteristics of CCHF patients stratified according to PON1gene polymorphisms, any statistically significant differences were not also observed (p > 0.05). Our study showed no possible association between genotypes of PON1 gene Q192R and L55M polymorphisms and CCHF.
克里米亚-刚果出血热（CCHF）是由克里米亚刚果出血热病毒（CCHFV）引起的急性病毒性出血热。对氧磷酶 1（PON1）是一种高密度脂蛋白（HDL）结合蛋白，可抵御身体氧化应激。为了研究 PON1 基因在 CCHF 中的作用，我们使用基于 PCR 的 RFLP 检测法，根据严重程度和死亡率对 CCHF 患者进行分层，筛选了两个单核苷酸多态性（Q192R [rs662]和 L55M [rs854560]）的基因型。总体而言，本研究纳入了 132 名确诊为 CCHF 的患者。Q192R 和 L55M 多态性的三种基因型和两种等位基因的频率在死亡率和疾病严重程度方面没有统计学上的显著差异（p＞0.05）。根据七种复合基因型，严重和轻度、致命和非致命 CCHF 患者之间也没有发现任何统计学上的显著差异（p＞0.05）。当我们根据 PON1 基因多态性对 CCHF 患者进行分层分析临床特征时，也没有观察到任何统计学上的显著差异（p＞0.05）。我们的研究表明，PON1 基因 Q192R 和 L55M 多态性的基因型与 CCHF 之间没有可能存在关联。
