Mukhtar Hayat Mohammad, Singh Amandeep, Soni Varinder, Singh Atinderpal, Kaur Hardeep
a Department of Pharmacognosy and Phytochemistry, SBS College of Pharmacy , Tarn Taran , India.
b Department of Pharmaceutical Sciences, IKG Punjab Technical University , Kapurthala , India.
Nat Prod Res. 2019 Oct;33(19):2868-2872. doi: 10.1080/14786419.2018.1503661. Epub 2018 Nov 23.
A simple, rapid, accurate and precise RP-HPLC method was developed for the determination of stigmasterol in botanical extract of . Separation was achieved with acetonitrile and acetic acid in water (75:25% v/v) in isocratic mode at 210 nm. Single sharp peak of standard stigmasterol was detected at retention time 3.17 min which overlay with the peak of plant extract at 3.14 min. The calibration curve was found to be linear in a concentration range of 2-10 μg/ml with correlation coefficient of 0.998. The LOD and LOQ were found to be 1.50 μg/ml and 4.55 μg/ml respectively. Accuracy and precision was determined with overall recovery of 99.6-100.1% for stigmasterol and RSD values in both intra-day and inter-day repeatability assay lesser than 0.340%, respectively. The robustness study also indicated that there is no influence of minor changes in detecting wavelength and flow rate of mobile phase on the response.
建立了一种简单、快速、准确且精密的反相高效液相色谱（RP-HPLC）方法，用于测定[植物提取物名称]中的豆甾醇。采用乙腈和水（75:25% v/v）中的乙酸在等度模式下于210 nm波长处实现分离。标准豆甾醇的单尖锐峰在保留时间3.17分钟处被检测到，其与植物提取物在3.14分钟处的峰重叠。校准曲线在2 - 10 μg/ml的浓度范围内呈线性，相关系数为0.998。检测限（LOD）和定量限（LOQ）分别为1.50 μg/ml和4.55 μg/ml。豆甾醇的准确度和精密度通过总体回收率99.6 - 100.1%来确定，日内和日间重复性试验中的相对标准偏差（RSD）值分别小于0.340%。稳健性研究还表明，检测波长和流动相流速的微小变化对响应没有影响。
