Department of Obstetrics and Gynecology, Hanchuan People's Hospital of Hubei Province, Hanchuan, China.
Eur Rev Med Pharmacol Sci. 2018 Nov;22(21):7105-7112. doi: 10.26355/eurrev_201811_16242.
The aim of this study was to investigate the role of long non-coding RNA (lncRNA) TCL6 in early abortion and to explore its underlying mechanism.
The expression levels of lncRNA-TCL6 and epidermal growth factor receptor (EGFR) in placental tissues of normal pregnancy, threatened abortion pregnancy, spontaneous abortion pregnancy, and induced abortion pregnancy were detected by quantitative Real-Time Polymerase Chain Reaction (qRT-PCR), respectively. Trophoblast cells were transfected with siRNA to knock-down lncRNA-TCL6. Cell viability of trophoblast cells was detected by cell counting kit-8 (CCK-8) assay. The protein expression levels of EGFR, extracellular regulated protein kinases (ERK) and protein kinase B (AKT) in trophoblast cells after lncRNA-TCL6 knockdown were detected by Western blot. Rescue experiments were performed to investigate the relationship between EGFR and lncRNA-TCL6.
The expression of lncRNA-TCL6 in placenta tissues of threatened abortion pregnancy was significantly higher than that of normal pregnancy. Meanwhile, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was also markedly higher than induced abortion pregnancy. However, the expression of EGFR showed an opposite trend. After knockdown of lncRNA-TCL6 in trophoblast cells, the protein expression levels of EGFR, ERK, and AKT were significantly increased when compared with those of the control group. CCK-8 assay indicated that cell viability was remarkably increased after knockdown of lncRNA-TCL6, which could be reversed by EGFR knockdown.
Compared with normal pregnancy, lncRNA-TCL6 was highly expressed in placental tissues of threatened abortion pregnancy. Moreover, the expression of lncRNA-TCL6 in placenta tissues of spontaneous abortion pregnancy was significantly higher than induced abortion pregnancy. Knockdown of lncRNA-TCL6 promoted the proliferation of trophoblast cells and inhibited the abortion via the EGFR signaling pathway.
本研究旨在探讨长链非编码 RNA（lncRNA）TCL6 在早期流产中的作用，并探讨其潜在机制。
采用实时定量聚合酶链反应（qRT-PCR）分别检测正常妊娠、先兆流产妊娠、自然流产妊娠和人工流产妊娠胎盘组织中 lncRNA-TCL6 和表皮生长因子受体（EGFR）的表达水平。用 siRNA 转染滋养细胞以敲低 lncRNA-TCL6。用细胞计数试剂盒-8（CCK-8）法检测滋养细胞的活力。用 Western blot 检测 lncRNA-TCL6 敲低后滋养细胞中 EGFR、细胞外调节蛋白激酶（ERK）和蛋白激酶 B（AKT）的蛋白表达水平。进行挽救实验以研究 EGFR 与 lncRNA-TCL6 之间的关系。
先兆流产妊娠胎盘组织中 lncRNA-TCL6 的表达明显高于正常妊娠。同时，自然流产妊娠胎盘组织中 lncRNA-TCL6 的表达也明显高于人工流产妊娠。然而，EGFR 的表达则呈现相反的趋势。在滋养细胞中敲低 lncRNA-TCL6 后，与对照组相比，EGFR、ERK 和 AKT 的蛋白表达水平显著增加。CCK-8 检测表明，敲低 lncRNA-TCL6 后细胞活力明显增加，而 EGFR 敲低则可逆转这一现象。
与正常妊娠相比，先兆流产妊娠胎盘组织中 lncRNA-TCL6 表达水平升高。此外，自然流产妊娠胎盘组织中 lncRNA-TCL6 的表达明显高于人工流产妊娠。敲低 lncRNA-TCL6 通过 EGFR 信号通路促进滋养细胞的增殖并抑制流产。
