Department of Radiotherapy, Gansu Provincial Cancer Hospital, Qilihe District, Lanzhou City, Gansu Province, China.
Eur Rev Med Pharmacol Sci. 2018 Nov;22(21):7305-7313. doi: 10.26355/eurrev_201811_16267.
LncRNA LINC00473 was reported to be upregulated in human cancers. Whereas, the role of LINC00473 in head and neck squamous cell carcinoma (HNSCC) and radiotherapy remains elusive.
Gene array analysis was performed to detect lncRNA LINC00473. Then, the expression of LINC00473 in HNSCC 78 pairs of tissues and cell lines was measured by qRT-PCR assay. To explore the detailed functions of LINC00473 on cell proliferation, MTT and colony formation assays were conducted. We also investigated the influence of LINC00473 expression on radioresistance of HNSCC cells. Western blot assay was used to confirm the relationship between LINC00473 and Wnt/β-catenin signaling pathway. Furthermore, the effects of x-ray treatment on LINC00473 expression and Wnt/β-catenin signaling pathway were detected by Western blot assay.
LncRNA LINC00473 was upregulated in HNSCC tissues and cell lines. Functional assays showed that LINC00473 acted as oncogene to promote cell proliferation and inhibit apoptosis. In addition, downregulation of LINC00473 enhanced the sensitivity of radiotherapy for HNSCC cells. Furthermore, Western blot assays demonstrated that Wnt/β-catenin signaling pathway was inhibited by LINC00473 knockdown. Notably, Western blot assay revealed that x-ray treatment suppressed the activity of Wnt/β-catenin signaling pathway after LINC00473 knockdown.
These data suggested that the upregulation of lncRNA LINC00473 promotes the radioresistance of HNSCC cells through activating Wnt/β-catenin signaling pathway.
长链非编码 RNA LINC00473 被报道在人类癌症中上调。然而，LINC00473 在头颈部鳞状细胞癌（HNSCC）和放疗中的作用仍不清楚。
进行基因芯片分析以检测 lncRNA LINC00473。然后，通过 qRT-PCR 检测 LINC00473 在 78 对 HNSCC 组织和细胞系中的表达。为了探讨 LINC00473 对细胞增殖的详细作用，进行了 MTT 和集落形成测定。我们还研究了 LINC00473 表达对 HNSCC 细胞放射抗性的影响。Western blot 测定用于证实 LINC00473 与 Wnt/β-catenin 信号通路之间的关系。此外，通过 Western blot 测定检测 X 射线处理对 LINC00473 表达和 Wnt/β-catenin 信号通路的影响。
LncRNA LINC00473 在 HNSCC 组织和细胞系中上调。功能测定表明，LINC00473 作为癌基因促进细胞增殖并抑制细胞凋亡。此外，下调 LINC00473 增强了 HNSCC 细胞对放疗的敏感性。此外，Western blot 测定表明，Wnt/β-catenin 信号通路被 LINC00473 敲低抑制。值得注意的是，Western blot 测定显示，X 射线处理在 LINC00473 敲低后抑制了 Wnt/β-catenin 信号通路的活性。
这些数据表明，长链非编码 RNA LINC00473 通过激活 Wnt/β-catenin 信号通路促进 HNSCC 细胞的放射抗性。
