Satoh T
Arch Microbiol. 1977 Dec 15;115(3):293-8. doi: 10.1007/BF00446455.
Effects of illumination on denitrification by a freshly isolated denitrifying phototrophic bacterium were investigated. Denitrification activity was induced when cells were grown in either light or darkness in the presence of nitrate without oxygen. Denitrification of nitrate with malate as the electron donor by cells at a phase of exponential growth occurred independently of illumination while that by cells in a stationary phase was activated. Effects of illumination on denitrification varied with electron donors. Using malate or succinate, denitrification by cells in a stationary phase was accelerated by illumination, inhibited when glucose or lactate was used, and independent of illumination when pyruvate was used. Denitrification by cells in an exponential phase was independent of illumination when succinate, malate or pyruvate was used and inhibited by it when glucose or lactate was used. Effects of illumination on the denitrification of nitrite were similar to those involving nitrate. Effects of various inhibitors on denitrification were examined in light-succinate and dark-lactate systems. Differences between the two systems are discussed.
研究了光照对新分离的反硝化光合细菌反硝化作用的影响。当细胞在无氧条件下于硝酸盐存在的情况下在光照或黑暗中生长时，反硝化活性被诱导。以苹果酸为电子供体时，处于指数生长期的细胞对硝酸盐的反硝化作用与光照无关，而处于稳定期的细胞的反硝化作用则被激活。光照对反硝化作用的影响因电子供体而异。使用苹果酸或琥珀酸时，光照可加速处于稳定期细胞的反硝化作用；使用葡萄糖或乳酸时，反硝化作用受到抑制；使用丙酮酸时，反硝化作用与光照无关。当使用琥珀酸、苹果酸或丙酮酸时，处于指数生长期的细胞的反硝化作用与光照无关，而使用葡萄糖或乳酸时则受到光照抑制。光照对亚硝酸盐反硝化作用的影响与对硝酸盐的影响相似。在光照 - 琥珀酸和黑暗 - 乳酸系统中研究了各种抑制剂对反硝化作用的影响。讨论了这两个系统之间的差异。
