Huang Liang, Zhao Peng, Liang Fei, Wang Wenhui
State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instruments, Tsinghua University, Beijing, China.
State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instruments, Tsinghua University, Beijing, China.
Methods Cell Biol. 2018;148:97-116. doi: 10.1016/bs.mcb.2018.06.013. Epub 2018 Jul 20.
Single-cell rotation is a fundamental manipulation used in a wide range of biotechnological applications such as cell injection and enucleation. However, there are currently few methods for the 3D rotation of single cells. Here, this chapter presents different biochip platforms based on a dielectrophoresis technique to achieve 3D rotation. In-plane (yaw) and out-of-plane rotation (pitch) can be achieved by applying different AC signal configurations, respectively. This use of 3D rotation facilitates several applications. For example, in-plane rotation can be used to measure the rotation spectra, and this can be used to estimate the dielectric parameters. The out-of-plane rotation can help reconstruct 3D cell models.
单细胞旋转是一种广泛应用于细胞注射和去核等多种生物技术应用中的基本操作。然而，目前用于单细胞三维旋转的方法很少。本章介绍了基于介电泳技术的不同生物芯片平台，以实现三维旋转。分别施加不同的交流信号配置可实现平面内（偏航）和平面外旋转（俯仰）。这种三维旋转的应用促进了多种应用。例如，平面内旋转可用于测量旋转光谱，并可用于估计介电参数。平面外旋转有助于重建三维细胞模型。
