Giriboni Julia, Gökdal Özdal, Eren Vadullah, Yaralı Engin, Santiago-Moreno Julián, Ungerfeld Rodolfo
Departamento de Fisiología, Facultad de Veterinaria, Universidad de la República, Montevideo, Uruguay.
Department of Plant and Animal Production, Çine Vocational School, Aydın Adnan Menderes University, Aydın, Turkey.
Anim Reprod Sci. 2019 Jan;200:43-50. doi: 10.1016/j.anireprosci.2018.11.009. Epub 2018 Nov 19.
The aim of this study was to determine if daily administration of a GnRH analogue (buserelin acetate) to bucks during the non-breeding season increases testosterone concentration and improves sperm quality. Five bucks received a daily dose of buserelin for 10 days, starting on Day 0 (first administration), and another five bucks remained as controls. Testosterone concentrations were greater in treated than in control bucks during the first hours after buserelin administration (P = 0.05), but greater in controls 10 h later (P < 0.01). Sperm mass motility and percentage of motile sperm were greater in treated (3.9 ± 0.6 and 70.1 ± 7.9%, respectively) than in control bucks (1.0 ± 0.6, P < 0.01; 45.0 ± 7.9%, P < 0.05 respectively) on Day 4. Percentage of sperm with normal morphology tended to be greater in treated than in control bucks (81.8 ± 6.2% compared with 63.5 ± 6.4% respectively, P = 0.08). The treatment decreased the percentage of sperm with mid piece defect and with bent tail (7.0 ± 1.5% compared with 12.0 ± 1.5%; 8.0 ± 1.7% compared with 13.5 ± 1.7%, treated and control bucks, respectively, P = 0.05 for both). The square root percentage of sperm with loose but heads with normal structures tended to be less in treated than control bucks (1.3 ± 0.3% compared with 0.4 ± 0.3% respectively, P = 0.06). It was concluded that daily administration of buserelin during the non-breeding season led to a rapid increase in testosterone concentration and improved sperm quality.
本研究的目的是确定在非繁殖季节对公羊每日给予促性腺激素释放激素类似物（醋酸布舍瑞林）是否会提高睾酮浓度并改善精子质量。五只公羊从第0天（首次给药）开始接受每日剂量的布舍瑞林，持续10天，另外五只公羊作为对照。布舍瑞林给药后的最初几小时内，治疗组公羊的睾酮浓度高于对照组（P = 0.05），但10小时后对照组的睾酮浓度更高（P < 0.01）。在第4天，治疗组公羊的精子总活力和活动精子百分比高于对照组（分别为3.9±0.6和70.1±7.9%）（对照组分别为1.0±0.6，P < 0.01；45.0±7.9%，P < 0.05）。治疗组公羊具有正常形态的精子百分比倾向于高于对照组（分别为81.8±6.2%和63.5±6.4%，P = 0.08）。治疗降低了中段缺陷和尾部弯曲精子的百分比（治疗组和对照组分别为7.0±1.5%和12.0±1.5%；8.0±1.7%和13.5±1.7%，两者P = 0.05）。头部结构正常但尾部松散的精子的平方根百分比在治疗组中倾向于低于对照组（分别为1.3±0.3%和0.4±0.3%，P = 0.06）。得出的结论是，在非繁殖季节每日给予布舍瑞林会导致睾酮浓度迅速升高并改善精子质量。
