Sato Kae, Sato Miwa, Yokoyama Mizuho, Hirai Mai, Furuta Aya
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University.
Anal Sci. 2019 Jan 10;35(1):49-56. doi: 10.2116/analsci.18SDP04. Epub 2018 Nov 23.
Microfluidic devices have emerged as a new cell culture tool, which can mimic the structure and physiology of living human organs. However, no standardized culture method for a microfluidic device has yet been established. Here, we describe the effects of various conditions on cell proliferation in a microchannel with a depth smaller than 100 μm. Primary endothelial cell proliferation was suppressed with a decrease in the culture medium volume per cell culture area. Moreover, cell growth was compared with or without medium flow, and the optimum culture condition was determined to be 1 μL/h flow in a 65-μm-deep microchannel. In addition, glucose consumption was greater under fluidic conditions than under static conditions, and the ability of tumor (HeLa) cells to convert glucose into lactate appeared to be higher in a static culture than that in a fluidic culture. Overall, our results will serve as a useful guide for designing a microfluidic cell culture platform in a channel smaller than 100 μm.
微流控装置已成为一种新型细胞培养工具，它能够模拟人体活体器官的结构和生理机能。然而，针对微流控装置，尚未建立标准化的培养方法。在此，我们描述了各种条件对深度小于100μm的微通道内细胞增殖的影响。随着每个细胞培养区域培养基体积的减少，原代内皮细胞的增殖受到抑制。此外，对有无培养基流动情况下的细胞生长进行了比较，确定最佳培养条件为在65μm深的微通道中以1μL/h的流速流动。另外，流体条件下的葡萄糖消耗量高于静态条件下的，并且肿瘤（HeLa）细胞将葡萄糖转化为乳酸的能力在静态培养中似乎高于流体培养。总体而言，我们的结果将为设计小于100μm通道的微流控细胞培养平台提供有益指导。
