Sato Kae, Maeda Momoko, Kamata Eriko, Ishii Sayaka, Yanagisawa Kanako, Kitajima Kenji, Hara Takahiko
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University, Bunkyo, Tokyo 112-8681, Japan.
Stem Cell Project, Tokyo Metropolitan Institute of Medical Science, Setagaya, Tokyo 156-8506, Japan.
Micromachines (Basel). 2020 Mar 14;11(3):305. doi: 10.3390/mi11030305.
A microfluidic co-culture system, consisting of mouse embryonic stem cells (mESCs)/OP9 cells, was evaluated as a platform for studying hematopoietic differentiation mechanisms in vitro. mESC differentiation into blood cells was achieved in a microchannel that had the minimum size necessary to culture cells. The number of generated blood cells increased or decreased based on the nitric oxide (NO) donor or inhibitor used. Conditioned medium from OP9 cell cultures also promoted an increase in the number of blood cells. The number of generated blood cells under normal medium flow conditions was lower than that observed under the static condition. However, when using a conditioned medium, the number of generated blood cells under flow conditions was the same as that observed under the static condition. We conclude that secreted molecules from OP9 cells have a large influence on the differentiation of mESCs into blood cells. This is the first report of a microfluidic mESC/OP9 co-culture system that can contribute to highly detailed hematopoietic research studies by mimicking the cellular environment.
一种由小鼠胚胎干细胞（mESCs）/OP9细胞组成的微流控共培养系统，被评估为体外研究造血分化机制的平台。在具有培养细胞所需最小尺寸的微通道中实现了mESC向血细胞的分化。产生的血细胞数量根据所使用的一氧化氮（NO）供体或抑制剂而增加或减少。OP9细胞培养的条件培养基也促进了血细胞数量的增加。正常培养基流动条件下产生的血细胞数量低于静态条件下观察到的数量。然而，当使用条件培养基时，流动条件下产生的血细胞数量与静态条件下观察到的数量相同。我们得出结论，OP9细胞分泌的分子对mESC向血细胞的分化有很大影响。这是关于微流控mESC/OP9共培养系统的首次报道，该系统通过模拟细胞环境有助于进行高度详细的造血研究。
