Yang Qing, Li Ying, Luo Lin
Department of Ophthalmology, Qingdao Municipal Hospital, Qingdao 266011, China.
Transl Neurosci. 2018 Nov 12;9:132-141. doi: 10.1515/tnsci-2018-0020. eCollection 2018.
Primary open angle glaucoma (POAG) is the most common form of glaucoma, with a multifactorial etiology that results in retinal ganglion cell death and loss of vision. In this study, we assessed the effects of myricetin on the trabecular meshwork cells in POAG.
In the model, glaucoma was induced in Sprague-Dawley rats by injecting hyaluronic acid into the anterior chamber of the eye (every week for six-weeks). Treatment group rats were administered myricetin (25, 50 or 100 mg/ kg body weight via oral gavage) each day for of six weeks.
POAG TM cells exposed to myricetin (25, 50 or 100 μM) exhibited significantly lowered reactive oxidative species (ROS) levels and lipid peroxidation products. The expressions of transforming growth factors (TGFβ1/β2), vascular endothelial growth factor, and senescence markers (senescence associated-β-galactosidase, cyclin-dependent kinase inhibitors-p16 and p21) were substantially down-regulated in POAG TM cells exposed to myricetin. Myricetin effectively prevented IOP elevation in glaucoma-induced rats and decreased inflammatory cytokines (IL-1α, IL-1β, IL-6, Il-8, TNF-α) in the aqueous humor and POAG TM cells of glaucoma-induced rats.
The observations of the study illustrate the protective effects of myricetin in glaucomatous TM cells.
原发性开角型青光眼（POAG）是最常见的青光眼类型，其病因多因素导致视网膜神经节细胞死亡和视力丧失。在本研究中，我们评估了杨梅素对POAG小梁网细胞的影响。
在模型中，通过向Sprague-Dawley大鼠眼前房注射透明质酸（每周一次，共六周）诱导青光眼。治疗组大鼠每天经口灌胃给予杨梅素（25、50或100mg/kg体重），持续六周。
暴露于杨梅素（25、50或100μM）的POAG小梁网细胞表现出活性氧（ROS）水平和脂质过氧化产物显著降低。在暴露于杨梅素的POAG小梁网细胞中，转化生长因子（TGFβ1/β2）、血管内皮生长因子和衰老标志物（衰老相关β-半乳糖苷酶、细胞周期蛋白依赖性激酶抑制剂p16和p21）的表达大幅下调。杨梅素有效预防了青光眼诱导大鼠的眼压升高，并降低了青光眼诱导大鼠房水和POAG小梁网细胞中的炎性细胞因子（IL-1α、IL-1β、IL-6、Il-8、TNF-α）。
该研究的观察结果表明杨梅素对青光眼小梁网细胞具有保护作用。
