Ruff M, Cavarelli J, Mikol V, Lorber B, Mitschler A, Giege R, Thierry J C, Moras D
Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France.
J Mol Biol. 1988 May 5;201(1):235-6. doi: 10.1016/0022-2836(88)90450-0.
Three new crystal forms of the complex between yeast tRNAAsp and aspartyl-tRNA synthetase have been produced. The best crystals, obtained after modifying both purification and crystallization conditions, belong to space group P2(1)2(1)2(1) and diffract to 2.7 A. Unit cell parameters are a = 210.4 A, b = 145.3 A and c = 86.0 A (1 A = 0.1 nm), with one dimeric enzyme and two tRNA molecules in the asymmetric unit.
已经产生了酵母天冬氨酸tRNA与天冬氨酰-tRNA合成酶复合物的三种新晶体形式。在对纯化和结晶条件进行改进后获得的最佳晶体属于空间群P2(1)2(1)2(1),衍射分辨率为2.7 Å。晶胞参数为a = 210.4 Å,b = 145.3 Å,c = 86.0 Å(1 Å = 0.1 nm),不对称单元中有一个二聚体酶和两个tRNA分子。