Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, 60455-900, Brazil.
Department of Biochemistry, Bahauddin Zakariya University, Multan, 60800, Pakistan.
Proteomics. 2019 Feb;19(3):e1800347. doi: 10.1002/pmic.201800347. Epub 2018 Dec 14.
Pollen grains are tiny structures vital for sexual reproduction and consequently seed and fruit production in angiosperms, and a source of many allergenic components responsible for deleterious implications for health worldwide. Current pollen research is mainly focused on unraveling the molecular mechanisms underlying the pollen germination and tube formation passing from the quiescent stage. In this context, an in-depth proteome analysis of the pollens from Ricinus communis at three different stages-that is, mature, hydrated, and in vitro germinated-is performed. This analysis results in the identification of 1950 proteins, including 1773, 1313, and 858, from mature, hydrated, and germinated pollens, respectively. Based on label-free quantification, 164 proteins are found to be significantly differentially abundant from mature to hydrated pollens, 40 proteins from hydrated to germinated, and 57 proteins from mature to germinated pollens, respectively. Most of the differentially abundant proteins are related to protein, carbohydrate, and energy metabolism and signaling. Besides other functional classes, a reasonable number of the proteins are predicted to be allergenic proteins, previously undiscovered. This is the first in-deep proteome analysis of the R. communis pollens and, to the best of our knowledge, one of the most complete proteome dataset identified from the pollens of any plant species, thus providing a reference proteome for researchers interested in pollen biology.
花粉粒是被子植物有性繁殖过程中至关重要的微小结构，对于种子和果实的产生起着关键作用，同时也是许多过敏原的来源，这些过敏原对全球的健康都有着不利的影响。目前，花粉的研究主要集中于揭示花粉从静止期到萌发和花粉管形成过程中的分子机制。在这一背景下，我们对蓖麻（Ricinus communis）的花粉在三个不同阶段（即成熟、水合和体外萌发）进行了深入的蛋白质组分析。这项分析共鉴定出 1950 种蛋白质，其中成熟花粉、水合花粉和萌发花粉中分别有 1773、1313 和 858 种蛋白质。基于无标记定量，我们发现 164 种蛋白质在成熟花粉到水合花粉之间的丰度存在显著差异，40 种蛋白质在水合花粉到萌发花粉之间的丰度存在差异，57 种蛋白质在成熟花粉到萌发花粉之间的丰度存在差异。大多数丰度差异显著的蛋白质与蛋白质、碳水化合物和能量代谢以及信号转导有关。除了其他功能类别，预测有相当数量的蛋白质是以前未发现的过敏原蛋白。这是对蓖麻花粉的首次深入蛋白质组分析，据我们所知，这也是从任何植物花粉中鉴定出的最完整的蛋白质组数据集之一，因此为对花粉生物学感兴趣的研究人员提供了一个参考蛋白质组。
