Wei J, Zhang M, Liang G, Li X
State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection, Henan Agricultural University, Zhengzhou, China.
Department of Entomology and BIO5 Institute, University of Arizona, Tucson, AZ, USA.
Insect Mol Biol. 2019 Jun;28(3):372-379. doi: 10.1111/imb.12556. Epub 2019 Jan 24.
Although membrane-bound alkaline phosphatases (ALPs) have been proposed as a receptor for Cry1Ac in a few lepidopteran species, their potential functions as a Cry2Ab receptor are yet to be verified. To determine if ALP2 also serves as a receptor for Cry1Ac and even for Cry2Ab in Helicoverpa zea, we measured the potency of activated Cry1Ac and Cry2Ab against midgut and fat body cell lines of H. zea and the ovarian cell line of Spodoptera frugiperda (Sf9) expressing H. zea ALP2 (HzALP2) or transfected with HzALP2 double-stranded RNA (dsRNA). Relative to the control cells, the three cell lines expressing HzALP2 were more susceptible to Cry1Ac but there was no difference for Cry2Ab. By contrast, the two H. zea cell lines transfected with HzALP2 dsRNA were resistant to Cry1Ac while kept susceptible to Cry2Ab. Furthermore, RNA interference knockdown of HzALP2 in H. zea larvae enhanced larval survival on Cry1Ac-containing diets. These findings indicate that HzALP2 functions as a receptor of Cry1Ac but not Cry2Ab.
尽管在一些鳞翅目昆虫中，膜结合碱性磷酸酶（ALPs）被认为是Cry1Ac的受体，但其作为Cry2Ab受体的潜在功能尚未得到证实。为了确定在玉米螟中，ALP2是否也是Cry1Ac甚至Cry2Ab的受体，我们测定了活化的Cry1Ac和Cry2Ab对玉米螟中肠和脂肪体细胞系以及表达玉米螟ALP2（HzALP2）或转染了HzALP2双链RNA（dsRNA）的草地贪夜蛾卵巢细胞系（Sf9）的效力。相对于对照细胞，表达HzALP2的三个细胞系对Cry1Ac更敏感，但对Cry2Ab没有差异。相比之下，用HzALP2 dsRNA转染的两个玉米螟细胞系对Cry1Ac具有抗性，而对Cry2Ab仍敏感。此外，RNA干扰敲低玉米螟幼虫中的HzALP2可提高幼虫在含Cry1Ac饲料上的存活率。这些发现表明，HzALP2是Cry1Ac的受体，但不是Cry2Ab的受体。
