长非编码 RNA Malat1 对于 T 细胞发育和对 LCMV 感染的反应并非必需。
Long noncoding RNA Malat1 is not essential for T cell development and response to LCMV infection.
机构信息
a Beijing Advanced Innovation Center for Food Nutrition and Human Health , China Agricultural University , Beijing , China.
b State Key Laboratory of Agrobiotechnology, College of Biological Sciences , China Agricultural University , Beijing , China.
出版信息
RNA Biol. 2018;15(12):1477-1486. doi: 10.1080/15476286.2018.1551705. Epub 2018 Dec 4.
Abstract
Long noncoding RNAs (lncRNAs) are emerging as critical mediators of various biological processes in the immune system. The current data showed that the lncRNA Malat1 is highly expressed in T cell subsets, but the function of Malat1 in T cell remains unclear. In this study, we detected the T cell development and both CD8 and CD4 T cell response to LCMV infection using Malat1 mice model. To our surprise, there were no significant defects in thymocytes at different developmental stages and the peripheral T cell pool with ablation of Malat1. During LCMV infection, Malat1 mice exhibited normal effector and memory CD8 T cells as well as T cells differentiation. Our results indicated that Malat1 is not essential for T cell development and T cell-mediated antiviral response though it expresses at very high level in different T cell populations.
摘要
长链非编码 RNA（lncRNAs）正在成为免疫系统中各种生物过程的关键调节因子。目前的数据表明，lncRNA Malat1 在 T 细胞亚群中高度表达，但 Malat1 在 T 细胞中的功能尚不清楚。在这项研究中，我们使用 Malat1 敲除小鼠模型检测了 T 细胞的发育以及 CD8 和 CD4 T 细胞对 LCMV 感染的反应。令我们惊讶的是，在不同发育阶段的胸腺细胞和外周 T 细胞库中，Malat1 的缺失并没有明显的缺陷。在 LCMV 感染期间，Malat1 敲除小鼠表现出正常的效应和记忆 CD8 T 细胞以及 T 细胞分化。我们的结果表明，尽管 Malat1 在不同的 T 细胞群体中高度表达，但它对于 T 细胞的发育和 T 细胞介导的抗病毒反应并非必需。
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