Faculty of Chemistry and Chemical Technology, University of Ljubljana, Večna pot 113, Ljubljana, Slovenia.
Center of Excellence for Biosensors, Instrumentation and Process Control - COBIK, Tovarniška cesta 26, Ajdovščina, Slovenia.
Appl Microbiol Biotechnol. 2019 Jan;103(2):685-694. doi: 10.1007/s00253-018-9527-y. Epub 2018 Nov 24.
High quantities of bacteriophages are currently used in the food industry and agriculture. However, growing antibiotic resistance of bacteria has recently awakened the interest to use bacteriophages for the treatment of bacterial infections in humans indicating that even higher quantities will be required in the future. High demand combined with a wide range of applications requires also efficient bacteriophage production processes operating at low production costs and with high productivity. To achieve this goal, different approaches were introduced and extensive studies of various parameters affecting bacteriophage formation were investigated. In this mini-review, we provide a short overview about different operation modes of bacteriophage production such as batch, semi-continuous and especially continuous with the pros and cons of each. We present factors affecting bacterial physiological state, its effect on phage formation and provide a description of methods for determination of bacteriophage growth parameters, through which bacteriophage formation is obtained. Understanding of described phenomena and inclusion of potential occurrence of mutations and selection in continuous systems enables evaluation of continuous process productivity and its optimization.
目前，大量噬菌体被应用于食品工业和农业。然而，随着细菌对抗生素耐药性的不断增强，噬菌体在人类细菌感染治疗方面的应用引起了人们的关注，这表明未来对噬菌体的需求将会更大。高需求加上广泛的应用，要求噬菌体的生产工艺具有高效率、低成本和高生产力。为了实现这一目标，人们引入了不同的方法，并对影响噬菌体形成的各种参数进行了广泛的研究。在这篇简要综述中，我们简要概述了噬菌体生产的不同操作模式，如分批、半连续和特别是连续，以及每种模式的优缺点。我们介绍了影响细菌生理状态的因素及其对噬菌体形成的影响，并提供了一种描述确定噬菌体生长参数的方法，通过该方法可以获得噬菌体的形成。对所描述现象的理解，并考虑连续系统中潜在的突变和选择的发生，使我们能够评估连续工艺的生产力并对其进行优化。
