Leibniz Institute for Natural Product Research and Infection Biology, Hans Knöll Institute, Beutenbergstr. 11a, 07745, Jena, Germany.
Institute for Inorganic and Analytical Chemistry, Friedrich Schiller University Jena, Lessingstrasse 8, 07743, Jena, Germany.
Biometals. 2019 Feb;32(1):77-88. doi: 10.1007/s10534-018-0159-6. Epub 2018 Nov 24.
Cupriachelin is a photoreactive lipopeptide siderophore produced by the freshwater bacterium Cupriavidus necator H16. In the presence of sunlight, the iron-loaded siderophore undergoes photolytic cleavage, thereby releasing solubilized iron into the environment. This iron is not only available to the siderophore producer, but also to the surrounding microbial community. In this study, the cupriachelin-based interaction between C. necator H16 and the freshwater diatom Navicula pelliculosa was investigated. A reporter strain of the bacterium was constructed to study differential expression levels of the cupriachelin biosynthesis gene cucJ in response to varying environmental conditions. Not only iron starvation, but also culture supernatants of N. pelliculosa were found to induce cupriachelin biosynthesis. The transcription factors involved in this differential gene expression were identified using DNA-protein pulldown assays. Besides the well-characterized ferric uptake regulator, a two-component system was found to tune the expression of cupriachelin biosynthesis genes in the presence of diatom supernatants.
铜绿假单胞菌素是一种由淡水细菌铜绿假单胞菌 H16 产生的光反应性脂肽类铁载体。在阳光的照射下，负载铁的铁载体发生光解断裂，从而将可溶铁释放到环境中。这种铁不仅可供铁载体生产者利用，也可供周围的微生物群落利用。在这项研究中，研究了铜绿假单胞菌素在铜绿假单胞菌 H16 和淡水硅藻皮壳藻之间的相互作用。构建了该细菌的报告株，以研究在不同环境条件下铜绿假单胞菌素生物合成基因 cucJ 的差异表达水平。不仅是铁饥饿，而且皮壳藻的培养液也被发现能诱导铜绿假单胞菌素的生物合成。使用 DNA-蛋白拉取测定法鉴定了参与这种差异基因表达的转录因子。除了特征明确的铁摄取调节剂外，还发现了一种二组分系统，在存在硅藻上清液的情况下调节铜绿假单胞菌素生物合成基因的表达。
