Fernández-Duran Irene, Tarrats Núria, Hari Priya, Acosta Juan Carlos
Edinburgh Cancer Research UK Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK.
Methods Mol Biol. 2019;1896:57-70. doi: 10.1007/978-1-4939-8931-7_7.
Inflammasomes are multimeric protein complexes that process IL-1β by cleaving the translated full-length protein into its active IL-1β mature fragment. In oncogene-induced senescence, inflammasomes play a crucial role by regulating IL1R signaling and consequently modulating proliferation and the senescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of IL1B expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.
炎性小体是多聚体蛋白复合物，通过将翻译后的全长蛋白切割成活性IL-1β成熟片段来加工IL-1β。在癌基因诱导的衰老中，炎性小体通过调节IL1R信号传导并因此调节增殖和衰老相关分泌表型（SASP）发挥关键作用。炎性小体激活需要两个步骤：(a) 通过激活IL1B表达引发炎性小体，随后(b) 成熟IL-1β的切割和释放。在本章中，我们描述了检测细胞衰老中炎性小体激活两个阶段的方法。
