University of Belgrade, Institute of Biological Research "Siniša Stanković", Department of Insect Physiology and Biochemistry, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
University of Belgrade, Institute of Biological Research "Siniša Stanković", Department of Insect Physiology and Biochemistry, Despot Stefan Blvd. 142, 11060 Belgrade, Serbia.
Chemosphere. 2019 Mar;218:416-424. doi: 10.1016/j.chemosphere.2018.11.112. Epub 2018 Nov 22.
Cadmium (Cd) can display a variety of different effects on living organisms. The objectives of the present study were to investigate Cd bioaccumulation and differences in parameters of oxidative stress (activities of the enzymes: superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase, glutathione S-transferase, and amounts of non-enzymatic free sulfhydryl groups and total glutathione) and cholinesterase activity in larval brain and midgut tissues of the polyphagous forest insect Lymantria dispar collected from unpolluted and polluted oak forests. Fourth instar L. dispar caterpillars from the unpolluted forest had higher body mass but accumulated more Cd in comparison to caterpillars from the polluted forest. In both populations the midgut was more sensitive than the brain to the prooxidative effects of Cd. Enzyme activities and amounts of non-enzymatic parameters tended to be greater in midgut tissues than in the brain, except for cholinesterase activity. Parameters of oxidative stress had higher values in caterpillar tissues from the polluted than from the unpolluted oak forest. The observed differences between the two natural populations point to the importance of knowing the history of population exposure to environmental pollution when monitoring forest ecosystems.
镉 (Cd) 对生物体可能会表现出多种不同的影响。本研究的目的是研究幼虫脑中 Cd 生物累积以及氧化应激参数（超氧化物歧化酶、过氧化氢酶、谷胱甘肽还原酶、抗坏血酸过氧化物酶、谷胱甘肽 S-转移酶的活性以及非酶类游离巯基和总谷胱甘肽的含量）和乙酰胆碱酯酶活性的差异，这些差异来自于取自未受污染和受污染栎树林的多寄主森林昆虫舞毒蛾（Lymantria dispar）幼虫。与来自污染森林的幼虫相比，未受污染森林中的 4 龄舞毒蛾幼虫具有更高的体重，但积累了更多的 Cd。在这两个种群中，与大脑相比，Cd 的促氧化作用对肠道更为敏感。除了乙酰胆碱酯酶活性之外，与大脑相比，肠道组织中的酶活性和非酶参数的含量往往更大。与未受污染的栎树林相比，来自污染栎树林的舞毒蛾幼虫组织中的氧化应激参数具有更高的值。这两个自然种群之间的观察到的差异表明，在监测森林生态系统时，了解种群暴露于环境污染的历史非常重要。
