Bjermer L, von Schoultz E, Norberg B, Henriksson R
Department of Oncology, University of Umeå, Sweden.
Prostate. 1988;13(1):49-55. doi: 10.1002/pros.2990130106.
Estramustine phosphate (EMP) influence on human monocyte phagocytosis of fluorescein isothiocyanate (FITC)-labeled yeast cells was measured in vitro. The method used, a modification of Hed's technique (FEMS Microbiol Lett 1:357, 1977), can differentiate between yeast cell engulfment and adherence to the phagocytotic cell surface. EMP is now accepted in the treatment of advanced prostatic carcinoma. In concentrations corresponding to the clinical situation (20-40 micrograms/ml), it dramatically inhibited the process of phagocytosis. The engulfment phase was inhibited, whereas cell adherence was less affected. This might be due to direct interaction with the microtubule system. The effects were totally reversible. In contrast, the metabolites estradiol and normustine did not affect engulfment of yeast cells, either as single agents or combined. The results demonstrate that the EMP complex caused an impaired phagocytosis, which could be of pathophysiological significance in the compromised cancer patients.
在体外测量了磷酸雌莫司汀（EMP）对人单核细胞吞噬异硫氰酸荧光素（FITC）标记的酵母细胞的影响。所采用的方法是对赫德技术（《FEMS微生物学快报》1:357，1977）的一种改进，该方法能够区分酵母细胞的吞噬作用和其在吞噬细胞表面的黏附。EMP目前已被用于晚期前列腺癌的治疗。在与临床情况相对应的浓度（20 - 40微克/毫升）下，它显著抑制了吞噬过程。吞噬阶段受到抑制，而细胞黏附则较少受到影响。这可能是由于与微管系统的直接相互作用。这些作用是完全可逆的。相比之下，代谢产物雌二醇和去甲氮芥无论是单独使用还是联合使用，都不会影响酵母细胞的吞噬作用。结果表明，EMP复合物导致吞噬作用受损，这在癌症患者身体机能受损的情况下可能具有病理生理学意义。
