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有氧暴露期间玉米青贮饲料的温度和微生物变化

Temperature and microbial changes of corn silage during aerobic exposure.

作者信息

Lee Seong Shin, Lee Hyuk Jun, Paradhipta Dimas Hand Vidya, Joo Young Ho, Kim Sang Bum, Kim Dong Hyeon, Kim Sam Churl

机构信息

Division of Applied Life Science (BK21Plus, Institute of Agriculture & Life Sciences), Gyeongsang National University, Jinju 52828, Korea.

Faculty of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.

出版信息

Asian-Australas J Anim Sci. 2019 Jul;32(7):988-995. doi: 10.5713/ajas.18.0566. Epub 2018 Nov 27.

Abstract

OBJECTIVE

This study was conducted to estimate the temperature and microbial changes of corn silages during aerobic exposure.

METHODS

Kwangpyeongok (KW) and Pioneer 1543 (PI) corn hybrids were harvested at 29.7% of dry matter and chopped to 3 to 5 cm lengths. Homo (Lactobacillus plantarum; LP) or hetero (Lactobacillus buchneri; LB) fermentative inoculants at 1.2×105 colony forming unit/g of fresh forage was applied to the chopped corn forage which was then ensiled in quadruplicate with a 2×2 (hybrid×inoculant) treatment arrangement for 100 days. After the silo was opened, silage was sub-sampled for analysis of chemical compositions, in vitro digestibility, and fermentation indices. The fresh silage was continued to determine aerobic exposure qualities by recorded temperature and microbial changes.

RESULTS

The KW silages had higher (p<0.01) in vitro digestibilities of dry matter and neutral detergent fiber than those of PI silages. Silages applied with LB had higher (p<0.001) acetate concentration, but lower (p<0.01) lactate concentration and lactate to acetate ratio than those of LP silages. The interaction effect among hybrid and inoculant was detected in acetate production (p = 0.008), aerobic stability (p = 0.006), and lactic acid bacteria count (p = 0.048). The yeast was lower (p = 0.018) in LB silages than that in LP silages. During the aerobic exposure, PI silages showed higher (p<0.05) temperature and mold than KW silages, while LP silages had higher (p<0.05) lactic acid bacteria and yeast than LB silages.

CONCLUSION

The results indicated that the changes of silage temperature during aerobic exposure seems mainly affected by mold growth, while applied LB only enhanced aerobic stability of PI silages.

摘要

目的

本研究旨在评估玉米青贮饲料在有氧暴露期间的温度和微生物变化。

方法

广坪玉(KW)和先锋1543(PI)玉米杂交种在干物质含量为29.7%时收获,并切碎至3至5厘米长。将植物乳杆菌(LP)或布氏乳杆菌(LB)发酵接种剂以1.2×10⁵ 菌落形成单位/克新鲜草料的量施用于切碎的玉米草料,然后将其以2×2(杂交种×接种剂)处理安排一式四份青贮100天。青贮窖打开后,对青贮饲料进行子样本采集,以分析化学成分、体外消化率和发酵指标。通过记录温度和微生物变化,继续对新鲜青贮饲料的有氧暴露质量进行测定。

结果

KW青贮饲料的干物质和中性洗涤纤维体外消化率高于PI青贮饲料(p<0.01)。施用LB的青贮饲料乙酸盐浓度较高(p<0.001),但乳酸盐浓度和乳酸盐与乙酸盐比率低于LP青贮饲料(p<0.01)。在乙酸盐产生(p = 0.008)、有氧稳定性(p = 0.006)和乳酸菌计数(p = 0.048)方面检测到杂交种和接种剂之间的交互作用。LB青贮饲料中的酵母菌数量低于LP青贮饲料(p = 0.018)。在有氧暴露期间,PI青贮饲料的温度和霉菌数量高于KW青贮饲料(p<0.

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0669/6601061/f723b41ae45e/ajas-18-0566f1.jpg

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