a Department of Anatomical Sciences, School of Medicine , Isfahan University of Medical Sciences , Isfahan , Iran.
b Department of Anatomical Sciences, School of Medicine , Tehran University of Medical Sciences , Tehran , Iran.
Nutr Cancer. 2018 Nov-Dec;70(8):1308-1314. doi: 10.1080/01635581.2018.1521438. Epub 2018 Nov 26.
The purpose of this study is evaluation the protective effect of omega3 on rat sperm protamination and ubiquitination after treatment with Bleomycin, Etoposide and Cisplatin (BEP) drugs. In present study, 40 male rats were divided into four groups: Control, BEP, Omega3 and BEP + Omega3. Sperm protamination and ubiquitination were assessed using chromomycin A3 and immunofluorescence staining respectively. The mean percentage of ubiquitinated sperm in BEP group was significantly increased relative to control group (P < .001). But, the mean percentage of sperm protamination significantly decreased in BEP group relative to control group (P < .001). Rats in BEP + Omega3 group showed a significantly decreased in the mean percentage of sperm ubiquitination as compared to BEP group (P < .05) while, sperm protamination increased significantly relative to BEP group (P < .001). Administration of Omega3 after chemotherapy showed an improvement in sperm ubiquitination and protamination. Our data indicated that omega3 after chemotherapy may be beneficial for chromatin remodeling during spermatogenesis following BEP treatment.
本研究旨在评估ω-3 对博来霉素、依托泊苷和顺铂（BEP）药物处理后大鼠精子鱼精蛋白和泛素化的保护作用。本研究中，将 40 只雄性大鼠分为四组：对照组、BEP 组、ω-3 组和 BEP+ω-3 组。分别采用 Chromomycin A3 和免疫荧光染色评估精子鱼精蛋白和泛素化。与对照组相比，BEP 组中泛素化精子的平均百分比显著增加（P<0.001）。但是，BEP 组精子鱼精蛋白的平均百分比与对照组相比显著降低（P<0.001）。与 BEP 组相比，BEP+ω-3 组的精子泛素化平均百分比显著降低（P<0.05），而精子鱼精蛋白则显著增加（P<0.001）。化疗后给予 ω-3 治疗可改善精子的泛素化和鱼精蛋白化。我们的数据表明，化疗后 ω-3 可能有益于 BEP 治疗后精子发生过程中的染色质重塑。
