Mukherjee S, Joardar N, Sinha Babu S P
Parasitology Laboratory, Department of Zoology (Centre for Advanced Studies), Visva-Bharati University, Santiniketan-731 235, India.
J Helminthol. 2018 Nov 27;94:e15. doi: 10.1017/S0022149X18001050.
A novel microfilarial sheath protein (MfP) of the human filarial parasite Wuchereria bancrofti and its proinflammatory activity on host macrophages were identified recently. MfP is a homolog of the nematode bestrophin-9 superfamily that acts as a ligand of macrophage Toll-like receptor 4 (TLR4) to induce inflammation through NF-κB activation. Therefore, the presence and functional implication of this novel protein in adult-stage parasites were open questions to answer. In this study, the bovine filarial parasite Setaria cervi was used to simulate adult W. bancrofti. We detected the presence of MfP in adult-stage S. cervi through clear immunological cross-reactivity and immunolocalization employing an anti-MfP antibody developed in mice. Therefore, our findings put forward S. cervi as a cost-effective source of immunodominant filarial antigen MfP to simulate its future utilization in the immunotherapeutic intervention of lymphatic filariasis.
最近鉴定出了人类丝虫寄生虫班氏吴策线虫的一种新型微丝蚴鞘蛋白（MfP）及其对宿主巨噬细胞的促炎活性。MfP是线虫bestrophin-9超家族的同源物，作为巨噬细胞Toll样受体4（TLR4）的配体，通过激活NF-κB诱导炎症。因此，这种新型蛋白在成虫阶段寄生虫中的存在及其功能意义是有待解答的问题。在本研究中，使用牛丝虫寄生虫鹿丝状线虫来模拟成年班氏吴策线虫。我们通过清晰的免疫交叉反应以及使用在小鼠中制备的抗MfP抗体进行免疫定位，检测到成虫阶段鹿丝状线虫中存在MfP。因此，我们的研究结果提出鹿丝状线虫作为免疫显性丝虫抗原MfP的一种经济有效的来源，以模拟其在淋巴丝虫病免疫治疗干预中的未来应用。
