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阿根廷西北部葡萄相关非酿酒酵母的种群及酿酒学特性

Population and oenological characteristics of non-Saccharomyces yeasts associated with grapes of Northwestern Argentina.

作者信息

Mendoza Lucía M, Vega-Lopez Guillermo A, Fernández de Ullivarri Miguel, Raya Raúl R

机构信息

Centro de Referencia para Lactobacilos (CERELA), Chacabuco 145, 4000, Tucumán, Argentina.

Facultad de Bioquímica, Química y Farmacia, Universidad Nacional de Tucumán, Ayacucho 491, 4000, Tucumán, Argentina.

出版信息

Arch Microbiol. 2019 Mar;201(2):235-244. doi: 10.1007/s00203-018-1601-4. Epub 2018 Nov 26.

Abstract

Yeasts population associated with grapes from Northwest Argentina, a region with a significant vine-growing increase over the past years, was evaluated. Ten species of non-Saccharomyces yeasts were identified from four grape varieties (Malbec, Merlot, Syrah and Torrontes) being Hanseniaspora uvarum the dominant species. Typing of isolates revealed genetic variability within Hanseniaspora genus and also high variability was observed according to their oenological characteristics. Based on the oenological properties, the most adequate strains as starter cultures were H. uvarum HuT7, HuMe15, HuS16, H. vineae HvT-mc1 and Metschnikowia pulcherrima MpT2/MpT3. These selected yeasts exhibited moderate resistance to SO, reduced values of volatile acidity, null or low production of HS, high levels of enzymes related to aroma and did not produce killer toxins. Further studies using mixed cultures of these non-Saccharomyces strains and S. cerevisiae are needed to validate the contribution of selected indigenous yeasts on wine organoleptic characteristics.

摘要

对来自阿根廷西北部葡萄的酵母菌群进行了评估,该地区在过去几年葡萄种植显著增加。从四个葡萄品种(马尔贝克、梅洛、西拉和托伦特斯)中鉴定出10种非酿酒酵母,其中葡萄汁有孢汉逊酵母是优势种。分离株分型显示有孢汉逊酵母属内存在遗传变异性,并且根据其酿酒特性也观察到高度变异性。基于酿酒特性,作为发酵剂培养的最合适菌株是葡萄汁有孢汉逊酵母HuT7、HuMe15、HuS16,葡萄有孢汉逊酵母HvT-mc1和美丽梅奇酵母MpT2/MpT3。这些选定的酵母对二氧化硫表现出中等抗性,挥发酸值降低,硫化氢产量为零或较低,与香气相关的酶水平较高,且不产生杀伤毒素。需要进一步研究这些非酿酒酵母菌株与酿酒酵母的混合培养,以验证选定的本土酵母对葡萄酒感官特性的贡献。

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