Krasnoperova E E, Isayenkov S V, Yemets A I, Blume Ya B
Tsitol Genet. 2016 Jul-Aug;50(4):3-10.
It was shown phenotypic changes in the root system of seedlings Arabidopsis thaliana in transgenic lines with overexpression and suppressed gene expression of serine-threonine protein kinase KIN10 in conditions of energy shortage and under normal conditions. The normal growth and development of KIN10 overexpressing plants with in energy deficiency conditions were detected. The significant inhibition of the plant development under normal conditions for these plant lines was obsereved. The levels of KIN10 gene expression under normal conditions in different organs A. thaliana, particularly in the roots, stems, leaves and flowers were analyzed. The highest level expression of the gene was fixed in the leaves.
结果表明,在能量短缺条件下和正常条件下,拟南芥幼苗根系在丝氨酸 - 苏氨酸蛋白激酶KIN10过表达和基因表达受抑制的转基因株系中出现了表型变化。检测了在能量缺乏条件下KIN10过表达植株的正常生长和发育情况。观察到这些株系在正常条件下植株发育受到显著抑制。分析了正常条件下拟南芥不同器官,特别是根、茎、叶和花中KIN10基因的表达水平。该基因的最高表达水平出现在叶片中。