Weetman R M, Chilcote R R, Rierden W J, Baehner R L
Exp Hematol. 1978 Jan;6(1):9-17.
Leukemic monoblasts obtained from three children were evaluated for their in vitro physiologic characteristics. These monoblasts were alpha naphthyl acetate esterase positive, exhibited glass adherence, had IgG membrane receptors, phagocytized latex particles with subsequent nitroblue tetrazolium (NBT) reduction, and matured to macrophages in vitro, either when adherent to glass slides or in feeder layer suspensions. Employing the double layer in vitro bone marrow culture technique, leukemic marrows from these children failed to produce colonies in culture in the presence or absence of normal feeder layers. Varying the concentration of leukemic cells in the feeder layers failed to augment colony growth from normal bone marrows. Although monoblasts were observed to mature to macrophages in vitro, no increase in colony stimulating factor activity was observed with aging of the feeder layers. These studies suggest that childhood leukemic monoblasts lack the ability to produce colony stimulating activity, differing in this respect from monoblastic leukemia in adults.
对从三名儿童身上获取的白血病原单核细胞的体外生理特性进行了评估。这些原单核细胞α-萘乙酸酯酶呈阳性，表现出玻璃黏附性，具有IgG膜受体，吞噬乳胶颗粒并随后使硝基蓝四氮唑（NBT）还原，并且在体外，无论是附着于载玻片上还是在饲养层悬浮液中，都能成熟为巨噬细胞。采用双层体外骨髓培养技术，这些儿童的白血病骨髓在有或没有正常饲养层的情况下均未能在培养中产生集落。改变饲养层中白血病细胞的浓度未能增强正常骨髓的集落生长。尽管观察到原单核细胞在体外成熟为巨噬细胞，但随着饲养层老化，未观察到集落刺激因子活性增加。这些研究表明，儿童白血病原单核细胞缺乏产生集落刺激活性的能力，在这方面与成人单核细胞白血病不同。
